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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23935

Title: Maternal intake of methyl-group donors affects DNA methylation of metabolic genes in infants
Authors: Pauwels, Sara
Ghosh, Manosij
Duca, Radu Corneliu
Bekaert, Bram
Freson, Kathleen
Huybrechts, Inge
Langie, Sabine A. S.
Koppen, Gudrun
Devlieger, Roland
Godderis, Lode
Issue Date: 2017
Publisher: BIOMED CENTRAL LTD
Citation: CLINICAL EPIGENETICS, 9, p. 1-13 (Art N° 16)
Abstract: Background: Maternal nutrition during pregnancy and infant nutrition in the early postnatal period (lactation) are critically involved in the development and health of the newborn infant. The Maternal Nutrition and Offspring's Epigenome (MANOE) study was set up to assess the effect of maternal methyl-group donor intake (choline, betaine, folate, methionine) on infant DNA methylation. Maternal intake of dietary methyl-group donors was assessed using a food-frequency questionnaire (FFQ). Before and during pregnancy, we evaluated maternal methyl-group donor intake through diet and supplementation (folic acid) in relation to gene-specific (IGF2 DMR, DNMT1, LEP, RXRA) buccal epithelial cell DNA methylation in 6 months old infants (n = 114) via pyrosequencing. In the early postnatal period, we determined the effect of maternal choline intake during lactation (in mothers who breast-fed for at least 3 months) on gene-specific buccal DNA methylation (n = 65). Results: Maternal dietary and supplemental intake of methyl-group donors (folate, betaine, folic acid), only in the periconception period, was associated with buccal cell DNA methylation in genes related to growth (IGF2 DMR), metabolism (RXRA), and appetite control (LEP). A negative association was found between maternal folate and folic acid intake before pregnancy and infant LEP (slope = -1.233, 95% CI -2.342; -0.125, p = 0.0298) and IGF2 DMR methylation (slope = -0.706, 95% CI -1.242; -0.107, p = 0.0101), respectively. Positive associations were observed for maternal betaine (slope = 0.875, 95% CI 0.118; 1.633, p = 0.0241) and folate (slope = 0.685, 95% CI 0.245; 1.125, p = 0.0027) intake before pregnancy and RXRA methylation. Buccal DNMT1 methylation in the infant was negatively associated with maternal methyl-group donor intake in the first and second trimester of pregnancy and negatively in the third trimester. We found no clear association between maternal choline intake during lactation and buccal infant DNA methylation. Conclusions: This study suggests that maternal dietary and supplemental intake of methyl-group donors, especially in the periconception period, can influence infant's buccal DNA methylation in genes related to metabolism, growth, appetite regulation, and maintenance of DNA methylation reactions.
Notes: [Pauwels, Sara; Ghosh, Manosij; Duca, Radu Corneliu; Godderis, Lode] Univ Leuven, KU Leuven, Dept Publ Hlth & Primary Care, Environm & Hlth, Kapucijnenvoer 35 Blok D Box 7001, B-3000 Leuven, Belgium. [Pauwels, Sara; Langie, Sabine A. S.; Koppen, Gudrun] Flemish Inst Technol Res VITO, Unit Environm Risk & Hlth, Boeretang 200, B-2400 Mol, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Dept Imaging & Pathol, B-3000 Leuven, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Univ Hosp Leuven, B-3000 Leuven, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Dept Forens Med, B-3000 Leuven, Belgium. [Bekaert, Bram] Univ Leuven, KU Leuven, Lab Forens Genet & Mol Archeol, B-3000 Leuven, Belgium. [Freson, Kathleen] Univ Leuven, KU Leuven, Ctr Mol & Vasc Biol, UZ Herestr 49,Box 911, B-3000 Leuven, Belgium. [Huybrechts, Inge] Int Agcy Res Canc, 150 Cours Albert Thomas, F-69372 Lyon 08, France. [Langie, Sabine A. S.] Hasselt Univ, Fac Sci, B-3590 Diepenbeek, Belgium. [Devlieger, Roland] Univ Leuven, KU Leuven, Dept Dev & Regenerat, B-3000 Leuven, Belgium. [Devlieger, Roland] Univ Hosp Leuven, Dept Obstet & Gynecol, B-3000 Leuven, Belgium. [Godderis, Lode] IDEWE, External Serv Prevent & Protect Work, Interleuvenlaan 58, B-3001 Heverlee, Belgium.
URI: http://hdl.handle.net/1942/23935
DOI: 10.1186/s13148-017-0321-y
ISI #: 000393925300002
ISSN: 1868-7083
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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