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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23770

Title: 3D full-field quantification of cell-induced large deformations in fibrillar biomaterials by combining non-rigid image registration with label-free second harmonic generation
Authors: Jorge-Peñas, Alvaro
Bové, Hannelore
Sanen, Kathleen
Vaeyens, Marie-Mo
Steuwe, Christian
Roeffaers, Maarten
Ameloot, Marcel
Van Oosterwyck, Hans
Issue Date: 2017
Citation: Biomaterials, 136, pag. 86-97
Status: In Press
Abstract: To advance our current understanding of cell-matrix mechanics and its importance for biomaterials development, advanced three-dimensional (3D) measurement techniques are necessary. Cell-induced deformations of the surrounding matrix are commonly derived from the displacement of embedded fiducial markers, as part of traction force microscopy (TFM) procedures. However, these fluorescent markers may alter the mechanical properties of the matrix or can be taken up by the embedded cells, and therefore influence cellular behavior and fate. In addition, the currently developed methods for calculating cell-induced deformations are generally limited to relatively small deformations, with displacement magnitudes and strains typically of the order of a few microns and less than 10% respectively. Yet, large, complex deformation fields can be expected from cells exerting tractions in fibrillar biomaterials, like collagen. To circumvent these hurdles, we present a technique for the 3D full-field quantification of large cell-generated deformations in collagen, without the need of fiducial markers. We applied non-rigid, Free Form Deformation (FFD)-based image registration to compute full-field displacements induced by MRC-5 human lung fibroblasts in a collagen type I hydrogel by solely relying on second harmonic generation (SHG) from the collagen fibrils. By executing comparative experiments, we show that comparable displacement fields can be derived from both fibrils and fluorescent beads. SHG-based fibril imaging can circumvent all described disadvantages of using fiducial markers. This approach allows measuring 3D full-field deformations under large displacement (of the order of 10 µm) and strain regimes (up to 40%). As such, it holds great promise for the study of large cell-induced deformations as an inherent component of cell-biomaterial interactions and cell-mediated biomaterial remodeling.
Notes: Van Oosterwyck, H (reprint author), Katholieke Univ Leuven, Dept Mech Engn, Celestijnenlaan 300C Box 2419, Leuven, Belgium. marcel.ameloot@uhasselt.be; hans.vanoosterwyck@kuleuven.be
URI: http://hdl.handle.net/1942/23770
DOI: 10.1016/j.biomaterials.2017.05.015
ISI #: 000401816500007
ISSN: 0142-9612
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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