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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23760

Title: A Tandem Green-Red Heterodimeric Fluorescent Protein with High FRET Efficiency
Authors: Wiens, Matthew D.
Shen, Yi
Li, Xi
Salem, M. Alaraby
Smisdom, Nick
Zhang, Wei
Brown, Alex
Campbell, Robert E.
Issue Date: 2016
Publisher: WILEY-V C H VERLAG GMBH
Citation: CHEMBIOCHEM, 17(24), p. 2361-2367
Abstract: The tetrameric red fluorescent protein from Discosoma sp. coral (DsRed) has previously been engineered to produce dimeric and monomeric fluorescent variants with excitation and emission profiles that span the visible spectrum. The brightest of the effectively monomeric DsRed variants is tdTomato-a tandem fusion of a dimeric DsRed variant. Here we describe the engineering of brighter red (RRvT), green (GGvT), and green-red heterodimeric (GRvT) tdTomato variants. GRvT exhibited 99% intramolecular FRET efficiency, resulting in long Stokes shift red fluorescence. These new variants could prove useful for multicolor live-cell imaging applications.
Notes: [Wiens, Matthew D.; Shen, Yi; Li, Xi; Salem, M. Alaraby; Smisdom, Nick; Zhang, Wei; Brown, Alex; Campbell, Robert E.] Univ Alberta, Dept Chem, 11227 Saskatchewan Dr, Edmonton, AB T6G 2G2, Canada. [Smisdom, Nick] Univ Hasselt, Fac Med & Life Sci, Martelarenlaan 42, B-3500 Hasselt, Belgium.
URI: http://hdl.handle.net/1942/23760
DOI: 10.1002/cbic.201600492
ISI #: 000392934100012
ISSN: 1439-4227
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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