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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23759

Title: Cell-Based Delivery of Interleukin-13 Directs Alternative Activation of Macrophages Resulting in Improved Functional Outcome after Spinal Cord Injury
Authors: Dooley, Dearbhaile
Lemmens, Evi
Vangansewinkel, Tim
Le Blon, Debbie
Hoornaert, Chloe
Ponsaerts, Peter
Hendrix, Sven
Issue Date: 2016
Publisher: CELL PRESS
Citation: STEM CELL REPORTS, 7(6), p. 1099-1115
Abstract: The therapeutic effects of mesenchymal stem cell (MSC) transplantation following spinal cord injury (SCI) to date have been limited. Therefore, we aimed to enhance the immunomodulatory properties of MSCs via continuous secretion of the anti-inflammatory cytokine interleukin-13 (IL-13). By using MSCs as carriers of IL-13 (MSC/IL-13), we investigated their therapeutic potential, compared with non-engineered MSCs, in a mouse model of SCI. We show that transplanted MSC/IL-13 significantly improve functional recovery following SCI, and also decrease lesion size and demyelinated area by more than 40%. Further histological analyses in CX(3)CR1(EGFP/+) CCR2(RFP/+) transgenic mice indicated that MSC/IL-13 significantly decrease the number of resident microglia and increase the number of alternatively activated macrophages. In addition, the number of macrophage-axon contacts in MSC/IL-13-treated mice was decreased by 50%, suggesting a reduction in axonal dieback. Our data provide evidence that transplantation of MSC/IL-13 leads to improved functional and histopathological recovery in a mouse model of SCI.
Notes: [Dooley, Dearbhaile; Lemmens, Evi; Vangansewinkel, Tim; Hendrix, Sven] Hasselt Univ, Biomed Res Inst, Dept Morphol, Martelarenlaan 42, B-3500 Hasselt, Belgium. [Dooley, Dearbhaile; Le Blon, Debbie; Hoornaert, Chloe; Ponsaerts, Peter] Univ Antwerp, Vaccine & Infect Dis Inst Vaxinfectio, Lab Expt Hematol, B-2610 Antwerp, Belgium.
URI: http://hdl.handle.net/1942/23759
DOI: 10.1016/j.stemcr.2016.11.005
ISI #: 000393188500008
ISSN: 2213-6711
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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