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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2364

Title: Osteoprotegerin and receptor activator of nuclear factor-kappa B ligand mRNA expression in patients with rheumatoid arthritis and healthy controls
Authors: VANDER BORGHT, Ann
LINSEN, Loes
THEWISSEN, Marielle
GEUSENS, Piet
RAUS, Jef
STINISSEN, Piet
Issue Date: 2004
Publisher: J RHEUMATOL PUBL CO
Citation: JOURNAL OF RHEUMATOLOGY, 31(8). p. 1483-1490
Abstract: Objective. To further understand the role of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappaB ligand (RANK-L) in rheumatoid arthritis (RA), we studied the levels of RANK-L and OPG mRNA in peripheral blood mononuclear cells (PBMC) and synovial tissue of patients with RA and controls. Methods. RANK-L and OPG mRNA levels were measured in PBMC and CD4+/CD8+ T cell subsets of patients with chronic RA, osteoarthritis (OA), and healthy controls, using quantitative real-time polymerase chain reaction. OPG and RANK-L mRNA levels were measured in paired blood and synovial tissue samples of patients with early, untreated RA at 2 timepoints with an interval of 16 weeks. Results. RANK-L mRNA levels were significantly higher in PBMC of patients with early and chronic RA compared to healthy controls. Contrary to healthy controls, RANK-L mRNA levels in patients with chronic RA were mainly of CD4+ T cell origin. OPG mRNA was observed in the blood of all (17/17) early RA patients, but could not be detected in chronic RA patients (0/14) or in patients with OA (0/8). Three out of 17 healthy controls showed measurable levels of OPG mRNA. The OPG/RANK-L ratio tended to be higher in the synovium than in the PBMC of early RA patients. RANK-L mRNA in synovial tissue was mainly of non-T cell origin. Conclusion. Since RANK-L and OPG mRNA levels are elevated in PBMC of RA patients, and CD4+ T cells are the major contributors to RANK-L mRNA expression, mononuclear cells in patients with RA may be involved in the pathways that regulate bone metabolism.
Notes: Limburgs Univ Ctr, Biomed Onderzoeksinst, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium.Stinissen, P, Limburgs Univ Ctr, Biomed Onderzoeksinst, Univ Campus, B-3590 Diepenbeek, Belgium.piet.stinissen@luc.ac.be
URI: http://hdl.handle.net/1942/2364
Link to publication: http://jrheum.com/abstracts/abstracts04/1483.html
ISI #: 000223004900005
ISSN: 0315-162X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2005
Appears in Collections: Research publications

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