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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2363

Title: Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines
Authors: Wang, P
Keijer, J
Bouwman, F
NOBEN, Jean-Paul
Issue Date: 2004
Citation: CELLULAR AND MOLECULAR LIFE SCIENCES, 61(18). p. 2405-2417
Abstract: Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20degreesC treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.
Notes: Maastricht Univ, Dept Human Biol, Nutr & Toxicol Res Inst Maastricht, Maastricht Prote Ctr, NL-6200 MD Maastricht, Netherlands. RIKILT Inst Food Safety, NL-6700 AE Wageningen, Netherlands. Limburgs Univ Ctr, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, B-3590 Diepenbeek, Belgium.Renes, J, Maastricht Univ, Dept Human Biol, Nutr & Toxicol Res Inst Maastricht, Maastricht Prote Ctr, POB 616, NL-6200 MD Maastricht, Netherlands.J.Renes@HB.unimaas.nl
URI: http://hdl.handle.net/1942/2363
DOI: 10.1007/s00018-004-4256-z
ISI #: 000224525900010
ISSN: 1420-682X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2005
Appears in Collections: Research publications

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