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|Title: ||Microdissection and SAGE as a combined tool to reveal gene expression in ductal carcinoma in situ of the breast|
|Authors: ||VERLINDEN, Ilse|
|Issue Date: ||2004|
|Citation: ||MOLECULAR CARCINOGENESIS, 41(4). p. 197-206|
|Abstract: ||The interplay between cancer cells and the normal surrounding tissue is believed to influence the biological behavior of the tumor. However, the presence of multiple cell types within the prelevated tumor specimen may attenuate changes that occur specifically in the malignant cells within their microenvironment. To study gene expression of the malignant cells in situ, we used a new microdissection method to separate ductal carcinoma in situ (DCIS) cells from the surrounding stroma, immunological infiltrates, and endothelial cells. We applied an adapted microSAGE protocol, without total mRNA amplification, to study their gene expression profile. Three thousand two hundred one different transcripts were identified in a total of 29 534 observed tags. of these unique tags, 88.3% matched known GenBank-sequences and 11.7% represented unknown transcripts. As compared to a total DCIS SAGE library, microdissection combined with SAGE revealed additional genes expressed only in normal surrounding, probably stromal, cells and not or significantly less in DCIS tumor cells. This study demonstrates that microdissection can be combined with SAGE as a tool to study transcriptomes. This approach provides important new information on differential gene expression both in tumor cells and normal surrounding tissue. Several of the observed differences indeed disappear when the total tumor mass is analyzed. (C) 2004 Wiley-Liss, Inc.|
|Notes: ||Transnatl Univ Limburg, Biomed Onderzoeksinst, Sch Life Sci, Limburgs Univ Ctr, B-3590 Diepenbeek, Belgium.Michiels, L, Transnatl Univ Limburg, Biomed Onderzoeksinst, Sch Life Sci, Limburgs Univ Ctr, Univ Campus Gebouw A, B-3590 Diepenbeek, Belgium.|
|ISI #: ||000225371600002|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2005|
|Appears in Collections: ||Research publications|
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