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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2332

Title: Effects of prolactin on cloned human T-Lymphocytes
Authors: Dogusan, Z
Martens, N
Demotte, N
Hooghe, R
Hooghe-Peters, EL
Issue Date: 2003
Citation: ENDOCRINE, 20(1-2). p. 171-175
Abstract: To evaluate the possible role of prolactin (PRL) in T-lymphocytes, we monitored gene induction in one cytotoxic T-lymphocyte (CTL) clone derived from a patient with hemochromatosis and in several T-helper clones generated from a normal donor and a patient with multiple sclerosis. The CTL clone expressed conventional PRL receptor (PRLR), and PRL induced the expression of suppressor of cytokine signaling-3 (SOCS-3) and increased the expression of SOCS-2 and cytokine-inducible src homology-2-containing protein (CIS, another member of the SOCS family). As is the case in granulocytes, expression of a conventional receptor for PRL could not be shown by polymerase chain reaction analysis on three helper clones. In addition, as in granulocytes, PRL modulated the expression of genes such as the interferon-regulatory factor-1, inducible nitric oxide synthase, CIS, and SOCS-2. These effects were also elicited with ovine PRL and could be prevented by anti-PRL antibodies. Thus, the use of clones allowed the detection of direct effects of PRL on T-cells, even when these have few or no detectable MR, confirming that human T-lymphocytes are targets for PRL.
Notes: Free Univ Brussels, Dept Pharmacol, FARC, Sch Med,Neuroendocrine Immunol Lab, B-1090 Brussels, Belgium. Limburgs Univ Ctr, Biomed Onderzoeksinst, Diepenbeek, Belgium. Ludwig Inst Canc Res, Brussels, Belgium.Hooghe-Peters, EL, Free Univ Brussels, Dept Pharmacol, FARC, Sch Med,Neuroendocrine Immunol Lab, Bldg D,Laarbeeklaan 103, B-1090 Brussels, Belgium.
URI: http://hdl.handle.net/1942/2332
ISI #: 000182483600022
ISSN: 0969-711X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2004
Appears in Collections: Research publications

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