Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23312

Title: Environmental exposure to human carcinogens in teenagers and the association with DNA damage
Authors: Franken, Carmen
Koppen, Gudrun
Lambrechts, Nathalie
Govarts, Eva
Bruckers, Liesbeth
Den Hond, Elly
Loots, Ilse
Nelen, Vera
Sioen, Isabelle
Nawrot, Tim S.
Baeyens, Willy
Van Larebeke, Nicolas
Boonen, Francis
Ooms, Daniella
Wevers, Mai
Jacobs, Griet
Covaci, Adrian
Schettgen, Thomas
Schoeters, Greet
Issue Date: 2017
Citation: ENVIRONMENTAL RESEARCH, 152, p. 165-174
Abstract: Background: We investigated whether human environmental exposure to chemicals that are labeled as (potential) carcinogens leads to increased (oxidative) damage to DNA in adolescents. Material and methods: Six hundred 14-15-year-old youngsters were recruited all over Flanders (Belgium) and in two areas with important industrial activities. DNA damage was assessed by alkaline and formamidopyrimidine DNA glycosylase (Fpg) modified comet assays in peripheral blood cells and analysis of urinary 8-hydroxydeoxyguanosine (8-OHdG) levels. Personal exposure to potentially carcinogenic compounds was measured in urine, namely: chromium, cadmium, nickel, 1-hydroxypyrene as a proxy for exposure to other carcinogenic polycyclic aromatic hydrocarbons (PAHs), t,t-muconic acid as a metabolite of benzene, 2,5-dichlorophenol (2,5-DCP), organophosphate pesticide metabolites, and di(2-ethylhexyl) phthalate (DEHP) metabolites. In blood, arsenic, polychlorinated biphenyl (PCB) congeners 118 and 156, hexachlorobenzene (HCB), dichlorodiphenyltrichloroethane (DDT) and perfluorooctanoic acid (PFOA) were analyzed. Levels of methylmercury (MeHg) were measured in hair. Multiple linear regression models were used to establish exposure-response relationships. Results: Biomarkers of exposure to PAHs and urinary chromium were associated with higher levels of both 8-OHdG in urine and DNA damage detected by the alkaline comet assay. Concentrations of 8-OHdG in urine increased in relation with increasing concentrations of urinary t,t-muconic acid, cadmium, nickel, 2,5-DCP, and DEHP metabolites. Increased concentrations of PFOA in blood were associated with higher levels of DNA damage measured by the alkaline comet assay, whereas DDT was associated in the same direction with the Fpg-modified comet assay. Inverse associations were observed between blood arsenic, hair MeHg, PCB 156 and HCB, and urinary 8-OHdG. The latter exposure biomarkers were also associated with higher fish intake. Urinary nickel and t,t-muconic acid were inversely associated with the alkaline comet assay. Conclusion: This cross-sectional study found associations between current environmental exposure to (potential) human carcinogens in 14-15-year-old Flemish adolescents and short-term (oxidative) damage to DNA. Prospective follow-up will be required to investigate whether long-term effects may occur due to complex environmental exposures.
Notes: [Franken, Carmen; Koppen, Gudrun; Lambrechts, Nathalie; Govarts, Eva; Den Hond, Elly; Boonen, Francis; Ooms, Daniella; Wevers, Mai; Jacobs, Griet; Schoeters, Greet] Flemish Inst Technol Res VITO, Mol, Belgium. [Franken, Carmen; Schoeters, Greet] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium. [Bruckers, Liesbeth] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium. [Loots, Ilse] Univ Antwerp, Polit & Social Sci, Antwerp, Belgium. [Nelen, Vera] Prov Inst Hyg, Antwerp, Belgium. [Sioen, Isabelle] Univ Ghent, Dept Publ Hlth, Ghent, Belgium. [Sioen, Isabelle] Univ Ghent, Dept Food Safety & Food Qual, Ghent, Belgium. [Nawrot, Tim S.] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium. [Nawrot, Tim S.] Leuven Univ, Dept Publ Hlth & Primary Care, Leuven, Belgium. [Baeyens, Willy; Van Larebeke, Nicolas] Vrije Univ Brussel, Dept Analyt & Environm Chem, Brussels, Belgium. [Van Larebeke, Nicolas] Univ Ghent, Dept Radiotherapy & Expt Cancerol, Ghent, Belgium. [Covaci, Adrian] Univ Antwerp, Dept Pharmaceut Sci, Toxicol Ctr, Antwerp, Belgium. [Schettgen, Thomas] Rhein Westfal TH Aachen, Dept Occupat & Social Med, Aachen, Germany. [Schoeters, Greet] Univ Southern Denmark, Inst Publ Hlth, Dept Environm Med, Odense, Denmark. [Den Hond, Elly] Prov Inst Hyg, Antwerp, Belgium.
URI: http://hdl.handle.net/1942/23312
DOI: 10.1016/j.envres.2016.10.012
ISI #: 000389684600021
ISSN: 0013-9351
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
Published version476.08 kBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.