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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23306

Title: Contrast Induced by a Static Magnetic Field for Improved Detection in Nanodiamond Fluorescence Microscopy
Authors: Singam, Shashi K. R.
Motylewski, Jaroslaw
Monaco, Antonina
Gjorgievska, Elena
Bourgeois, Emilie
Nesladek, Milos
Giugliano, Michele
Goovaerts, Etienne
Issue Date: 2016
Publisher: AMER PHYSICAL SOC
Citation: PHYSICAL REVIEW APPLIED, 6(6) (Art N° 064013)
Abstract: Diamond nanoparticles with negatively charged nitrogen-vacancy (NV) centers are highly efficient nonblinking emitters that exhibit spin-dependent intensity. An attractive application of these emitters is background-free fluorescence microscopy exploiting the fluorescence quenching induced either by resonant microwaves (RMWs) or by an applied static magnetic field (SMF). Here, we compare RMW-and SMF-induced contrast measurements over a wide range of optical excitation rates for fluorescent nanodiamonds (FNDs) and for NV centers shallowly buried under the (100)-oriented surface of a diamond single crystal (SC). Contrast levels are found to be systematically lower in the FNDs than in the SC. At low excitation rates, the RMW contrast initially rises to a maximum (up to 7% in FNDs and 13% in the SC) but then decreases steadily at higher intensities. Conversely, the SMF contrast increases from approximately 12% at low excitation rates to high values of 20% and 38% for the FNDs and SC, respectively. These observations are well described in a rate-equations model for the charged NV defect using parameters in good agreement with the literature. The SMF approach yields higher induced contrast in image collection under commonly applied optical excitation. Unlike the RMW method, there is no thermal load exerted on the aqueous media in biological samples in the SMF approach. We demonstrate imaging by SMF-induced contrast in neuronal cultures incorporating FNDs (i) in a setup for patch-clamp experiments in parallel with differential-interference-contrast microscopy, (ii) after a commonly used staining procedure as an illustration of the high selectivity against background fluorescence, and (iii) in a confocal fluorescence microscope in combination with bright-field microscopy.
Notes: [Singam, Shashi K. R.; Goovaerts, Etienne] Univ Antwerp, Dept Phys, Univ Pl 1, BE-2610 Antwerp, Belgium. [Motylewski, Jaroslaw; Monaco, Antonina; Giugliano, Michele] Univ Antwerp, Dept Biomed Sci, Theoret Neurobiol & Neuroengn Lab, Univ Pl 1, BE-2610 Antwerp, Belgium. [Gjorgievska, Elena; Bourgeois, Emilie; Nesladek, Milos] Univ Hasselt, Inst Mat Res IMO, Wetenschapspk 1, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/23306
DOI: 10.1103/PhysRevApplied.6.064013
ISI #: 000391036500001
ISSN: 2331-7019
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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