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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23250

Title: Arabidopsis plants exposed to gamma radiation in two successive generations show a different oxidative stress response
Authors: van de Walle, Jorden
Horemans, Nele
Saenen, Eline
Van Hees, May
Wannijn, Jean
Nauts, Robin
van Gompel, Axel
Vangronsveld, Jaco
Vandenhove, Hildegarde
Cuypers, Ann
Issue Date: 2016
Publisher: ELSEVIER SCI LTD
Citation: JOURNAL OF ENVIRONMENTAL RADIOACTIVITY, 165, p. 270-279
Abstract: When terrestrial environments get contaminated with long-lived gamma emitting radionuclides, plants that grow in these contaminated areas are exposed to gamma radiation during consecutive generations. Therefore it is important to evaluate the gamma induced stress response in plants in and between generations. The objective of this research is to reveal differences at the level of the antioxidative stress response between generations with a different radiation history. An experiment was conducted in which 7-days old Arabidopsis thaliana plants were exposed for 14 days to four different gamma dose rates: 22 mGy/h, 38 mGy/h, 86 mGy/h and 457 mGy/h. Two different plant groups were used: plants that were not exposed to gamma radiation before (P0) and plants that received the aforementioned gamma treatment during their previous generation (S1). Growth, the concentration of the antioxidants ascorbate and glutathione, a number of antioxidative enzyme activities and their gene transcript levels were analysed. A dose-rate dependent induction was seen for catalase (CAT) and guaiacol peroxidase (GPX) in the roots and for syringaldazine peroxidase (SPX) in the shoots. Differences between the two generations were observed for CAT and GPX in the roots, where a significantly higher activity of these reactive oxygen species (ROS) detoxifying enzymes was observed in the S1 generation. For SPX in the shoots, a dose dependent upregulation was observed in the PO generation. However, high SPX activities were present for all doses in the S1 generation. These differences in enzyme activity between generations for SPX and GPX and the involvement of these enzymes in cell wall biosynthesis, suggest an important role for cell wall strengthening in the response to gamma irradiation. (C) 2016 Elsevier Ltd. All rights reserved.
Notes: [van de Walle, Jorden; Horemans, Nele; Saenen, Eline; Van Hees, May; Wannijn, Jean; Nauts, Robin; van Gompel, Axel; Vandenhove, Hildegarde] SCK CEN, Belgian Nucl Res Ctr, Mol, Belgium. [van de Walle, Jorden; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/23250
DOI: 10.1016/j.jenvrad.2016.10.014
ISI #: 000390500100030
ISSN: 0265-931X
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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