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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/23233

Title: Visceral Leishmaniasis Patients Display Altered Composition and Maturity of Neutrophils as well as Impaired Neutrophil Effector Functions
Authors: Yizengaw, Endalew
Getahun, Mulusew
Tajebe, Fitsumbrhan
Cervera, Edward Cruz
Adem, Emebet
Mesfin, Getnet
Hailu, Asrat
Van der Auwera, Gert
Yardley, Vanessa
Lemma, Mulualem
Shkedy, Ziv
Diro, Ermias
Yeshanew, Arega
Melkamu, Roma
Mengesha, Bewketu
Modolell, Manuel
Munder, Markus
Müller, Ingrid
Takele, Yegnasew
Kropf, Pascale
Issue Date: 2016
Publisher: FRONTIERS MEDIA SA
Citation: FRONTIERS IN IMMUNOLOGY, 7 (Art N° 517)
Abstract: Immunologically, active visceral leishmaniasis (VL) is characterized by profound immunosuppression, severe systemic inflammatory responses, and an impaired capacity to control parasite replication. Neutrophils are highly versatile cells, which play a crucial role in the induction as well as the resolution of inflammation, the control of pathogen replication, and the regulation of immune responses. Neutrophil functions have been investigated in human cutaneous leishmaniasis; however, their role in human VL is poorly understood. In the present study we evaluated the activation status and effector functions of neutrophils in patients with active VL and after successful anti-leishmanial treatment. Our results show that neutrophils are highly activated and have degranulated; high levels of arginase, myeloperoxidase, and elastase, all contained in neutrophils' granules, were found in the plasma of VL patients. In addition, we show that a large proportion of these cells are immature. We also analyzed effector functions of neutrophils that are essential for pathogen clearance and show that neutrophils have an impaired capacity to release neutrophil extracellular traps, produce reactive oxygen species, and phagocytose bacterial particles, but not Leishmania parasites. Our results suggest that impaired effector functions, increased activation, and immaturity of neutrophils play a key role in the pathogenesis of VL.
Notes: [Yizengaw, Endalew; Getahun, Mulusew; Tajebe, Fitsumbrhan; Mesfin, Getnet] Univ Gondar, Dept Immunol, Gondar, Ethiopia. [Cervera, Edward Cruz; Mueller, Ingrid; Takele, Yegnasew; Kropf, Pascale] Imperial Coll London, Dept Med, London, England. [Adem, Emebet; Diro, Ermias; Yeshanew, Arega; Melkamu, Roma; Mengesha, Bewketu; Takele, Yegnasew] Gondar Univ, Leishmaniasis Res & Treatment Ctr, Gondar, Ethiopia. [Hailu, Asrat] Univ Addis Ababa, Dept Microbiol Immunol & Parasitol, Addis Ababa, Ethiopia. [Van der Auwera, Gert] Inst Trop Med, Dept Biomed Sci, Antwerp, Belgium. [Yardley, Vanessa] London Sch Hyg & Trop Med, Dept Immunol & Infect, London, England. [Lemma, Mulualem] Univ Gondar, Dept Internal Med, Gondar, Ethiopia. [Skhedy, Ziv] Univ Hasselt, Dept Math & Stat, Hasselt, Belgium. [Modolell, Manuel] Max Planck Inst Immunobiol & Epigenet, Dept Cellular Immunol, Freiburg, Germany. [Munder, Markus] Univ Med Ctr Mainz, Dept Med Hematol Oncol & Pneumol 3, Mainz, Germany. [Yizengaw, Endalew] Bahir Dar Univ, Dept Microbiol Immunol & Parasitol, Bahir Dar, Ethiopia. [Getahun, Mulusew] Ethiopian Publ Hlth Inst, Addis Ababa, Ethiopia. [Mesfin, Getnet] Ethiopian Publ Hlth Inst, Addis Ababa, Ethiopia.
URI: http://hdl.handle.net/1942/23233
DOI: 10.3389/fimmu.2016.00517
ISI #: 000388749300001
ISSN: 1664-3224
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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