www.uhasselt.be
DSpace

Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/22761

Title: Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms
Authors: Plusquin, Michelle
De Mulder, Katrien
Van Belleghem, Frank
DeGheselle, Olivier
Pirotte, Nicky
Willems, Maxime
Cuypers, Ann
Salvenmoser, Willi
Ladurner, Peter
Artois, Tom
Smeets, Karen
Issue Date: 2016
Publisher: WILEY-BLACKWELL
Citation: ENVIRONMENTAL TOXICOLOGY, 31(10), p. 1217-1228
Abstract: Stem cells or undifferentiated cells can cope more easily with external stresses. To evaluate the impact of toxic compounds on stem cell dynamics in vivo, in relation to other biological responses, we use the carcinogenic element cadmium and the regenerating model organism Macrostomum lignano. Through both BrdU and anti-histone H3 immunostainings, cadmium-induced effects were investigated at different stages of the stem cell cycle. A 24-h exposure to 100 and 250 M CdCl2 significantly decreased the number of stem cells (neoblasts) in mitosis, whereas the number of cells in the S phase remained unchanged. After this short-term exposure, the ultrastructure of the neoblasts was minimally affected in contrast to the epidermal tissues. These results were supported by gene expression data: transcripts of cdc2 and pig3 were significantly upregulated during all treatments. Both genes are involved in the cell cycle progression and are transcribed in the gonadal region, where stem cells are highly represented. Based on a substantial increase in gene expression of heat shock proteins (HSP) and their high activity in the gonadal region, we hypothesize that these proteins are key players in the protection of stem cells against external stresses. Apart from the strong HSP induction, other protective processes including cell division, apoptosis and anti-oxidative defence, were also activated. We, therefore, conclude that the protection of stem cells against external stressors may be based on the interplay between stem cell maintenance, i.e. repair and recovery through division, on one hand and apoptosis on the other hand.
Notes: [Plusquin, Michelle; Van Belleghem, Frank; DeGheselle, Olivier; Pirotte, Nicky; Cuypers, Ann; Artois, Tom; Smeets, Karen] Hasselt Univ, Dept Biol, Ctr Environm Sci, Agoralaan, Diepenbeek, Belgium. [De Mulder, Katrien; Salvenmoser, Willi; Ladurner, Peter] Univ Innsbruck, Inst Zool, Ctr Mol Biosci Innsbruck, Innsbruck, Austria. [Willems, Maxime] Univ Ghent, Pharmaceut Technol, St Pietersnieuwstr, Ghent, Belgium. [Van Belleghem, Frank] Open Univ, Fac Management Sci & Technol, Valkenburgerweg 177, Heerlen, Netherlands.
URI: http://hdl.handle.net/1942/22761
DOI: 10.1002/tox.22129
ISI #: 000383611900006
ISSN: 1520-4081
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
Published version708.94 kBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.