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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2276

Title: Morphological and immunocytochernical characterization of cultured fibroblast-like cells derived from adult human synovial membrane
Authors: VANDENABEELE, Frank
De Bari, C
MOREELS, Marjan
LAMBRICHTS, Ivo
Dell'Accio, F
LIPPENS, Peter
Luyten, FP
Issue Date: 2003
Publisher: JAPAN SOC HISTOL DOCUMENTATION NIIGATA UNIV MEDICAL SCHOOL
Citation: ARCHIVES OF HISTOLOGY AND CYTOLOGY, 66(2). p. 145-153
Abstract: The synovial membrane (SM) is a source of multipotent mesenchymal stem cells (MSCs), which appeared microscopically to be a relatively homogeneous population of fibroblast-like cells (FCs) in culture (De Bari et al., 2001). The aim of this study was to investigate phenotypic characteristics of the SM-derived FCs (SD-FCs) that could elucidate their origin inside the synovial tissue. Morphological characterization of SD-FCs was assessed by electron microscopy and by expression of surfactant protein A (SPA). This study, yielded substantial evidence that SD-FCs show ultrastructural and immunocytochemical features of type B synoviocytes; they contained characteristic lamellar bodies (LBs) that are secreted by exocytosis. LB secretion ability was maintained upon passaging (P3-P10). Immunocytochemistry showed that SD-FCs express surfactant protein A (SP-A). Taken together, these results indicate that multipotent SD-MSCs may originate from the synovial lining, having a phenotype highly similar to that of type B synoviocytes. We believe our data highlight the potent ability of type B synoviocytes to have a multilineage differentiation potential.
Notes: Limburgs Univ Ctr, DWI, Inst Biomed Res, Histol Lab, B-3590 Diepenbeek, Belgium. Katholieke Univ Leuven, Lab Skeletal Dev & Joint Disorders Onderwijs & Na, Louvain, Belgium.Vandenabeele, F, Limburgs Univ Ctr, DWI, Inst Biomed Res, Histol Lab, Univ Campus, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/2276
DOI: 10.1679/aohc.66.145
ISI #: 000183586600003
ISSN: 0914-9465
Category: A1
Type: Journal Contribution
Validation: ecoom, 2004
Appears in Collections: Research publications

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