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|Title: ||Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 degrees C|
|Authors: ||Stancescu, Maria|
Fedotova, Tatiana A.
Kolpashchikov, Dmitry M.
|Issue Date: ||2016|
|Publisher: ||AMER CHEMICAL SOC|
|Citation: ||JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138(41), p. 13465-13468|
|Abstract: ||Detection of point mutations and single nucleotide polymorphisms in DNA and RNA has a growing importance in biology, biotechnology, and medicine. For the application at hand, hybridization assays are often used. Traditionally, they differentiate point mutations only at elevated temperatures (>40 degrees C) and in narrow intervals (Delta T = 1-10 degrees C). The current study demonstrates that a specially designed multistranded DNA probe can differentiate point mutations in the range of 5-40 degrees C. This unprecedentedly broad ambient-temperature range is enabled by a controlled combination of (i) nonequilibrium hybridization conditions and (ii) a mismatch-induced increase of equilibration time in respect to that of a fully matched complex, which we dub "kinetic inversion".|
|Notes: ||[Stancescu, Maria; Fedotova, Tatiana A.; Kolpashchikov, Dmitry M.] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Natl Ctr Forens Sci, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Burnett Sch Biomed Sci, Orlando, FL 32816 USA. [Hooyberghs, Jef] VITO, Flemish Inst Technol Res, Boeretang 200, B-2400 Mol, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Campus Diepenbeek,Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Balaeff, Alexander] NanoSci Technol Ctr, 12424 Res Pkwy,Suite 400, Orlando, FL 32826 USA.|
|ISI #: ||000385992100004|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2017|
|Appears in Collections: ||Research publications|
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