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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/22747

Title: Nonequilibrium Hybridization Enables Discrimination of a Point Mutation within 5-40 degrees C
Authors: Stancescu, Maria
Fedotova, Tatiana A.
Hooyberghs, Jef
Balaeff, Alexander
Kolpashchikov, Dmitry M.
Issue Date: 2016
Citation: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138(41), p. 13465-13468
Abstract: Detection of point mutations and single nucleotide polymorphisms in DNA and RNA has a growing importance in biology, biotechnology, and medicine. For the application at hand, hybridization assays are often used. Traditionally, they differentiate point mutations only at elevated temperatures (>40 degrees C) and in narrow intervals (Delta T = 1-10 degrees C). The current study demonstrates that a specially designed multistranded DNA probe can differentiate point mutations in the range of 5-40 degrees C. This unprecedentedly broad ambient-temperature range is enabled by a controlled combination of (i) nonequilibrium hybridization conditions and (ii) a mismatch-induced increase of equilibration time in respect to that of a fully matched complex, which we dub "kinetic inversion".
Notes: [Stancescu, Maria; Fedotova, Tatiana A.; Kolpashchikov, Dmitry M.] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Natl Ctr Forens Sci, Orlando, FL 32816 USA. [Kolpashchikov, Dmitry M.] Univ Cent Florida, Burnett Sch Biomed Sci, Orlando, FL 32816 USA. [Hooyberghs, Jef] VITO, Flemish Inst Technol Res, Boeretang 200, B-2400 Mol, Belgium. [Hooyberghs, Jef] Hasselt Univ, Theoret Phys, Campus Diepenbeek,Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. [Balaeff, Alexander] NanoSci Technol Ctr, 12424 Res Pkwy,Suite 400, Orlando, FL 32826 USA.
URI: http://hdl.handle.net/1942/22747
DOI: 10.1021/jacs.6b05628
ISI #: 000385992100004
ISSN: 0002-7863
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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