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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/22728

Title: Heads or Tails: Genotyping of Hepatitis C Virus Concerning the 2k/1b Circulating Recombinant Form
Authors: Schuermans, Wim
Orlent, Hans
Desombere, Isabelle
Descheemaeker, Patrick
Van Vlierberghe, Hans
Geerts, Anja
Verhelst, Xavier
Reynders, Marijke
Padalko, Elizaveta
Issue Date: 2016
Publisher: MDPI AG
Abstract: As different hepatitis C virus (HCV) genotypes respond differently to initiated therapy, correct HCV genotyping is essential. A potential risk for misclassification of the intergenotypic HCV circulating recombinant form (CRF) 2k/1b strains exists, depending on the genotyping method used. The aim was to investigate the differences in HCV genotyping methods with regard to CRF 2k/1b and to gain insight in the prevalence of the CRF 2k/1b. Genotyping results by Versant HCV Genotype Assay were compared with nonstructural protein 5B (NS5B) sequencing. In total, from November 2001 until March 2015, 3296 serum samples were analyzed by Versant HCV Genotype Assay. As misclassified CRF is harbored among HCV genotype 2, we further focused our search on 142 (4.3%) samples positive for HCV genotype 2. On 116 (81.7%) retrieved samples, the NS5B sequencing was performed. Twelve out of the 116 retrieved samples (10.3%) were classified as CRF 2k/1b by sequencing of the NS5B region. Ten of these 12 samples were originally misclassified as genotype 2a or 2c, while 2 of them were misclassified as genotype 2. Our results show that the current prevalence of CRF 2k/1b is underestimated. The importance of correct HCV genotyping is emphasized, considering the tailored choice of treatment regimen and overall prognosis.
Notes: [Schuermans, Wim; Padalko, Elizaveta] Ghent Univ & Hosp, Dept Clin Chem Microbiol & Immunol, B-9000 Ghent, Belgium. [Orlent, Hans] AZ Sint Jan Bruges Ostend, Dept Gastroenterol & Hepatol, B-8000 Brugge, Belgium. [Desombere, Isabelle] Ghent Univ & Hosp, Ctr Vaccinol, B-9000 Ghent, Belgium. [Descheemaeker, Patrick; Reynders, Marijke] AZ Sint Jan Bruges Ostend, Dept Lab Med, Clin Microbiol, B-8000 Brugge, Belgium. [Van Vlierberghe, Hans; Geerts, Anja; Verhelst, Xavier] Ghent Univ & Hosp, Dept Gastroenterol & Hepatol, B-8000 Ghent, Belgium. [Padalko, Elizaveta] Hasselt Univ, Sch Life Sci, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/22728
DOI: 10.3390/ijms17091384
ISI #: 000385525500012
ISSN: 1422-0067
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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