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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/22623

Title: Transient loading of CD34+ hematopoietic progenitor cells with polystyrene nanoparticles
Authors: Deville, Sarah
Hadiwikarta, Wahyu Wijaya
Smisdom, Nick
Wathiong, Bart
Ameloot, Marcel
Nelissen, Inge
Hooyberghs, Jef
Issue Date: 2016
Citation: International Journal of Nanomedicine, 12, pag. 459-472
Status: In Press
Abstract: CD34+ hematopoietic progenitor cells (HPCs) offer great opportunities to develop new treatments for numerous malignant and non-malignant diseases. Nanoparticle (NP)-based strategies can further enhance this potential, therefore a thorough understanding of the loading behavior of HPCs towards NPs is essential to a successful application. Present study focusses on the interaction kinetics of 40-nm sized carboxylated polystyrene (PS) NPs with HPCs. Interestingly, a transient association of the NPs with HPCs is observed, reaching a maximum within one hour and declining afterwards. This behavior is not seen in dendritic cells (CD34-DCs) differentiated from HPCs, which display a monotonic increase in NP load. We demonstrate that this transient interaction requires an energy-dependent cellular process, suggesting active loading and release of NPs by HPCs. This novel observation offers a unique approach to transiently equip HPCs. A simple theoretical approach modelling NP loading and release is presented, contributing to a framework to describe this phenomenon.
Notes: Hooyberghs, J (reprint author), Flemish Inst Technol Res, Boeretang 200, B-2400 Mol, Belgium. jef.hooyberghs@vito.be
URI: http://hdl.handle.net/1942/22623
DOI: 10.2147/IJN.S119407
ISI #: 000391767100001
ISSN: 1178-2013
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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