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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/22595

Title: Cardiac atrial appendage stem cells promote angiogenesis in vitro and in vivo
Authors: Fanton, Yanick
Houbrechts, Cynthia
Willems, Leen
Daniëls, Annick
Linsen, Loes
Ratajczak, Jessica
Bronckaers, Annelies
Lambrichts, Ivo
Declercq, Jeroen
Rummens, Jean-Luc
Hendrikx, Marc
Hensen, Karen
Issue Date: 2016
Abstract: Cardiac atrial appendage stem cells (CASCs) show extraordinary myocardial differentiation properties, making them ideal candidates for myocardial regeneration. However, since the myocardium is a highly vascularized tissue, revascularization of the ischemic infarct area is essential for functional repair. Therefore, this study assessed if CASCs contribute to cardiac angiogenesis via paracrine mechanisms. First, it was demonstrated that CASCs produce and secrete high levels of numerous angiogenic growth factors, including vascular endothelial growth factor (VEGF), endothelin-1 (ET-1) and insulin-like growth factor binding protein 3 (IGFBP-3). Functional in vitro assays with a human microvascular endothelial cell line (HMEC-1) and CASC CM showed that CASCs promote endothelial cell proliferation, migration and tube formation, the most important steps of the angiogenesis process. Addition of inhibitory antibodies against identified growth factors could significantly reduce these effects, indicating their importance in CASC-induced neovascularization. The angiogenic potential of CASCs and CASC CM was also confirmed in a chorioallantoic membrane assay, demonstrating that CASCs promote blood vessel formation in vivo. In conclusion, this study shows that CASCs not only induce myocardial repair by cardiomyogenic differentiation, but also stimulate blood vessel formation by paracrine mechanisms. The angiogenic properties of CASCs further strengthen their therapeutic potential and make them an optimal stem cell source for the treatment of ischemic heart disease.
Notes: [Fanton, Yanick; Willems, Leen; Daniels, Annick; Declercq, Jeroen; Rummens, Jean-Luc; Hensen, Karen] Jessa Hosp, Lab Expt Hematol, Hasselt, Belgium. [Fanton, Yanick; Houbrechts, Cynthia; Willems, Leen; Declercq, Jeroen; Rummens, Jean-Luc; Hendrikx, Marc; Hensen, Karen] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium. [Linsen, Loes] Univ Hosp Leuven, AC Biobanking, Leuven, Belgium. [Ratajczak, Jessica; Bronckaers, Annelies; Lambrichts, Ivo] Hasselt Univ, Biomed Res Inst, Hasselt, Belgium. [Hendrikx, Marc] Jessa Hosp, Dept Cardiothorac Surg, Hasselt, Belgium.
URI: http://hdl.handle.net/1942/22595
DOI: 10.1016/j.yjmcc.2016.06.005
ISI #: 000382800100026
ISSN: 0022-2828
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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