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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/22075

Title: Imaging of transfection and intracellular release of intact, non-labeled DNA using fluorescent nanodiamonds
Authors: Petrakova, V.
Benson, V.
Buncek, M.
Fiserova, A.
Ledvina, M.
Stursa, J.
Cigler, P.
Nesladek, Milos
Issue Date: 2016
Citation: NANOSCALE, 8(23), p. 12002-12012
Abstract: Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor. The proposed device innovates the strategies for NA imaging and delivery, by providing detection of the intracellular release of non-labeled NAs without affecting cellular processing of the NAs. Our system highlights the potential of nanodiamonds to act not merely as labels but also as non-toxic and non-photobleachable fluorescent bio-sensors reporting complex molecular events.
Notes: [Petrakova, V.; Benson, V.; Fiserova, A.; Ledvina, M.; Nesladek, M.] Czech Tech Univ, Fac Biomed Engn, Sitna Sq 3105, Kladno 27201, Czech Republic. [Petrakova, V.] Inst Phys AS CR, Vvi, Slovance 1999-2, Prague 18221 8, Czech Republic. [Benson, V.; Fiserova, A.] Inst Microbiol AS CR, Vvi, Videnska 1083, Prague 14220 4, Czech Republic. [Buncek, M.] Generi Biotech Ltd, Machkova 587, Hradec Kralove 50011, Czech Republic. [Ledvina, M.; Cigler, P.] Inst Organ Chem & Biochem AS CR, Vvi, Flemingovo Nam 2, Prague 16610 6, Czech Republic. [Stursa, J.] Nucl Phys Inst AS CR, Vvi, Rez 25068, Czech Republic. [Nesladek, M.] Hasselt Univ, IMEC Div IMOMEC, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. [Nesladek, M.] Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/22075
DOI: 10.1039/c6nr00610h
ISI #: 000378510700029
ISSN: 2040-3364
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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