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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2198

Title: Product identification and adenylyl cyclase activity in chloroplasts of Nicotiana tabacum
Authors: Witters, E
QUANTEN, Lieve
BLOEMEN, Jo
VALCKE, Roland
Van Onckelen, H
Issue Date: 2004
Publisher: JOHN WILEY & SONS LTD
Citation: RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 18(4). p. 499-504
Abstract: In view of the ongoing debate on plant cyclic nucleotide metabolism, especially the functional presence of adenylyl cyclase, a novel detection method has been worked out to quantify the reaction product. Using uniformly labelled N-15-ATP as a substrate for adenylyl cyclase, a qualitative and quantitative liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI-MS/MS) method was developed to measure de novo formed N-15-adenosine 3',5'-cyclic monophosphate. Adenylyl cyclase activity was observed in chloroplasts obtained from Nicotiana tabacum cv. Petit Havana and the kinetic parameters and influence of various metabolic effectors are discussed in their context. Copyright (C) 2004 John Wiley Sons, Ltd.
Notes: Univ Antwerp, Dept Biol, Lab Plant Biochem & Plant Physiol, B-2610 Antwerp, Belgium. Limburgs Univ Ctr, Lab Plant Physiol, Dept SBG BGE, B-3590 Diepenbeek, Belgium.Witters, E, Univ Antwerp, Dept Biol, Lab Plant Biochem & Plant Physiol, Univ Pl 1, B-2610 Antwerp, Belgium.erwin.witters@ua.ac.be
URI: http://hdl.handle.net/1942/2198
DOI: 10.1002/rcm.1365
ISI #: 000189088700018
ISSN: 0951-4198
Category: A1
Type: Journal Contribution
Validation: ecoom, 2005
Appears in Collections: Research publications

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