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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21835

Title: Impedimetric immunosensor for the detection of histamine based on reduced graphene oxide
Authors: Delle, Lotta E.
Huck, Christina
Baecker, Matthias
Mueller, Frank
Grandthyll, Samuel
Jacobs, Karin
Lilischkis, Rainer
Vu, Xuan T.
Schoening, Michael J.
Wagner, Patrick
Thoelen, Ronald
Weil, Maryam
Ingebrandt, Sven
Issue Date: 2015
Abstract: In this work, we present a reduced graphene oxide-based impedimetric histamine biosensor. Histamine as a bioamine mediates immune-modulatory effects in acute inflammatory and allergic reactions. In a proof-of-principle approach, the low-cost sensor platform determines low concentrations of histamine in buffer solution with an impedimetric readout technique. As a sensor platform we used ultra-thin graphene oxide lines fabricated by a simple soft-lithographic approach. The lines of about 20m width were characterised by atomic force microscopy and scanning electron microscopy. Reduction of graphene oxide to a conductive transducer material was performed by a rapid thermal protocol in inert atmosphere. The reduction process was monitored by impedance spectroscopy and X-ray photoelectron spectroscopy. For our biosensor experiments we adopted a commonly-used protocol for surface plasmon resonance (SPR) detection of histamine. With our reduced graphene oxide platform we detected antigen-antibody binding events of histamine with concentrations of 0.1-1M via impedance changes in a low frequency range. The results from the rGO sensors were comparable to the SPR detection.
Notes: [Delle, Lotta E.; Lilischkis, Rainer; Vu, Xuan T.; Weil, Maryam; Ingebrandt, Sven] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst Technol, D-66482 Zweibrucken, Germany. [Huck, Christina; Baecker, Matthias; Schoening, Michael J.] FH Aachen, INB, D-52428 Julich, Germany. [Mueller, Frank; Grandthyll, Samuel; Jacobs, Karin] Univ Saarland, Expt Phys, D-66123 Saarbrucken, Germany. [Vu, Xuan T.] Rhein Westfal TH Aachen, Dept Phys IA, D-52074 Aachen, Germany. [Wagner, Patrick] Katholieke Univ Leuven, Dept Soft Matter & Biophys, B-3001 Leuven, Belgium. [Wagner, Patrick; Thoelen, Ronald] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/21835
DOI: 10.1002/pssa.201431863
ISI #: 000356706500022
ISSN: 1862-6300
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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