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|Title: ||Impedimetric immunosensor for the detection of histamine based on reduced graphene oxide|
|Authors: ||Delle, Lotta E.|
Vu, Xuan T.
Schoening, Michael J.
|Issue Date: ||2015|
|Publisher: ||WILEY-V C H VERLAG GMBH|
|Citation: ||PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 212 (6), p. 1327-1334|
|Abstract: ||In this work, we present a reduced graphene oxide-based impedimetric histamine biosensor. Histamine as a bioamine mediates immune-modulatory effects in acute inflammatory and allergic reactions. In a proof-of-principle approach, the low-cost sensor platform determines low concentrations of histamine in buffer solution with an impedimetric readout technique. As a sensor platform we used ultra-thin graphene oxide lines fabricated by a simple soft-lithographic approach. The lines of about 20m width were characterised by atomic force microscopy and scanning electron microscopy. Reduction of graphene oxide to a conductive transducer material was performed by a rapid thermal protocol in inert atmosphere. The reduction process was monitored by impedance spectroscopy and X-ray photoelectron spectroscopy. For our biosensor experiments we adopted a commonly-used protocol for surface plasmon resonance (SPR) detection of histamine. With our reduced graphene oxide platform we detected antigen-antibody binding events of histamine with concentrations of 0.1-1M via impedance changes in a low frequency range. The results from the rGO sensors were comparable to the SPR detection.|
|Notes: ||[Delle, Lotta E.; Lilischkis, Rainer; Vu, Xuan T.; Weil, Maryam; Ingebrandt, Sven] Univ Appl Sci Kaiserslautern, Dept Informat & Microsyst Technol, D-66482 Zweibrucken, Germany. [Huck, Christina; Baecker, Matthias; Schoening, Michael J.] FH Aachen, INB, D-52428 Julich, Germany. [Mueller, Frank; Grandthyll, Samuel; Jacobs, Karin] Univ Saarland, Expt Phys, D-66123 Saarbrucken, Germany. [Vu, Xuan T.] Rhein Westfal TH Aachen, Dept Phys IA, D-52074 Aachen, Germany. [Wagner, Patrick] Katholieke Univ Leuven, Dept Soft Matter & Biophys, B-3001 Leuven, Belgium. [Wagner, Patrick; Thoelen, Ronald] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium.|
|ISI #: ||000356706500022|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2016|
|Appears in Collections: ||Research publications|
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