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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21795

Title: Experimental evidence for proteins constituting virion components and particle morphogenesis of bacteriophage ZF40
Authors: Korol, Natalia
Van den Bossche, An
Romaniuk, Liudmyla
Noben, Jean-Paul
Lavigne, Rob
Tovkach, Fedor
Issue Date: 2016
Abstract: Bacteriophage ZF40 is the only currently available, temperate Myoviridae phage infecting the potato pathogen Pectobacterium carotovorum subsp. carotovorum. Despite its unusual tail morphology, its major tail sheath and tube proteins remained uncharacterized after the initial genome annotation. Using ESI tandem mass-spectrometry, 24 structural proteins of the ZF40 virion were identified, with a sequence coverage ranging between 15.8% and 87.8%. The putative function of 16 proteins could be elucidated based on secondary structure analysis and conservative domain searches. The experimental annotation of 35% of the encoded gene products within the structural region of the genome represents a complete view of the virion structure, which can serve as the basis for future structural analysis as a model phage.
Notes: [Korol, Natalia; Tovkach, Fedor] NAS Ukraine, DK Zabolotny Inst Microbiol & Virol, Dept Bacteriophage Mol Genet, UA-D03680 Kiev, Ukraine. [Van den Bossche, An; Lavigne, Rob] Katholieke Univ Leuven, Dept Biosyst, Lab Gene Technol, B-3001 Leuven, Belgium. [Van den Bossche, An] Sci Inst Publ Hlth WIV ISP, Div Bacterial Dis, B-1050 Brussels, Belgium. [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Transnatl Univ Limburg, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/21795
DOI: 10.1093/femsle/fnw042
ISI #: 000377967000013
ISSN: 0378-1097
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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