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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21706

Title: Prenatal Ambient Air Pollution, Placental Mitochondrial DNA Content, and Birth Weight in the INMA (Spain) and ENVIRONAGE (Belgium) Birth Cohorts
Authors: Clemente, Diana B. P.
Casas, Maribel
Vilahur, Nadia
Begiristain, Haizea
Bustamante, Mariona
Carsin, Anne-Elie
Fernandez, Mariana F.
Fierens, Frans
Gyselaers, Wilfried
Iniguez, Carmen
Janssen, Bram G.
Lefebvre, Wouter
Llop, Sabrina
Olea, Nicolas
Pedersen, Marie
Pieters, Nicky
Santa Marina, Loreto
Souto, Ana
Tardon, Adonina
Vanpoucke, Charlotte
Vrijheid, Martine
Sunyer, Jordi
Nawrot, Tim S.
Issue Date: 2016
Citation: ENVIRONMENTAL HEALTH PERSPECTIVES, 124 (5), p. 659-665
Abstract: BACKGROUND: Mitochondria are sensitive to environmental toxicants due to their lack of repair capacity. Changes in mitochondrial DNA (mtDNA) content may represent a biologically relevant intermediate outcome in mechanisms linking air pollution and fetal growth restriction. OBJECTIVE: We investigated whether placental mtDNA content is a possible mediator of the association between prenatal nitrogen dioxide (NO2) exposure and birth weight. METHODS: We used data from two independent European cohorts: INMA (n=376; Spain) and ENVIRONAGE (n=550; Belgium). Relative placental mtDNA content was determined as the ratio of two mitochondrial genes (MT-ND1 and MTF3212/R3319) to two control genes (RPLP0 and ACTB). Effect estimates for individual cohorts and the pooled data set were calculated using multiple linear regression and mixed models. We also performed a mediation analysis. RESULTS: Pooled estimates indicated that a 10-mu g/m(3) increment in average NO2 exposure during pregnancy was associated with a 4.9% decrease in placental mtDNA content (95% CI: -9.3, -0.3%) and a 48-g decrease (95% CI: -87, -9 g) in birth weight. However, the association with birth weight was significant for INMA (-66g; 95% CI: -111, -23g) but not for ENVIRONAGE (-20 g; 95% CI: -101, 62 g). Placental mtDNA content was associated with significantly higher mean birth weight (pooled analysis, interquartile range increase: 140 g; 95% CI: 43, 237 g). Mediation analysis estimates, which were derived for the INMA cohort only, suggested that 10% (95% CI: 6.6, 13.0 g) of the association between prenatal NO2 and birth weight was mediated by changes in placental mtDNA content. CONCLUSION: Our results suggest that mtDNA content can be one of the potential mediators of the association between prenatal air pollution exposure and birth weight.
Notes: [Clemente, Diana B. P.; Janssen, Bram G.; Pieters, Nicky; Nawrot, Tim S.] Hasselt Univ, Ctr Environm Sci, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium. [Clemente, Diana B. P.; Casas, Maribel; Bustamante, Mariona; Carsin, Anne-Elie; Pedersen, Marie; Vrijheid, Martine; Sunyer, Jordi] Ctr Res Environm Epidemiol CREAL, Barcelona, Spain. [Casas, Maribel; Bustamante, Mariona; Fernandez, Mariana F.; Iniguez, Carmen; Llop, Sabrina; Olea, Nicolas; Pedersen, Marie; Santa Marina, Loreto; Souto, Ana; Tardon, Adonina; Vrijheid, Martine; Sunyer, Jordi] Inst Hlth Carlos III, CIBERESP, Madrid, Spain. [Vilahur, Nadia] Karolinska Inst, Inst Environm Med IMM, S-10401 Stockholm, Sweden. [Begiristain, Haizea; Vrijheid, Martine; Sunyer, Jordi] Hlth Res Inst BIODONOSTIA, Gipuzkoa, Spain. [Bustamante, Mariona] Univ Pompeu Fabra, Barcelona, Spain. [Bustamante, Mariona; Santa Marina, Loreto] CRG, Barcelona, Spain. [Fernandez, Mariana F.; Olea, Nicolas] Univ Granada, Dept Radiol, Granada, Spain. [Fernandez, Mariana F.; Olea, Nicolas] Hosp Univ San Cecilio, Ibs GRANADA, Inst Invest Biosanitaria Granada, Granada, Spain. [Fierens, Frans; Vanpoucke, Charlotte] Belgian Interreg Environm Agcy, Brussels, Belgium. [Gyselaers, Wilfried] East Limburg Hosp, Dept Obstet, Genk, Belgium. [Iniguez, Carmen; Llop, Sabrina] Fdn Promot Hlth & Biomed Res Valencian Reg FISABI, Valencia, Spain. [Iniguez, Carmen] Univ Valencia, Valencia, Spain. [Lefebvre, Wouter] Flemish Inst Technol Res VITO, Mol, Belgium. [Pedersen, Marie] INSERM Natl Inst Hlth & Med Res, U823, Team Environm Epidemiol Appl Reprod & Resp Hlth, Inst Albert Bonniot, Grenoble, France. [Souto, Ana; Tardon, Adonina] Univ Oviedo, Univ Inst Oncol, Mol Epidemiol Canc Unit, Oviedo, Spain. [Sunyer, Jordi] Hosp del Mar, Res Inst, IMIM, Barcelona, Spain. [Nawrot, Tim S.] Katholieke Univ Leuven, Unit Environm & Hlth, Dept Publ Hlth & Primary Care, Leuven, Belgium.
URI: http://hdl.handle.net/1942/21706
DOI: 10.1289/ehp.1408981
ISI #: 000377077000023
ISSN: 0091-6765
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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