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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21611

Title: Cellular Interferon Gamma and Granzyme B Responses to Cytomegalovirus-pp65 and Influenza N1 Are Positively Associated in Elderly
Authors: Theeten, Heidi
Mathei, Catharina
Peeters, Kelly
Ogunjimi, Benson
Goossens, Herman
Ieven, Margareta
Van Damme, Pierre
Cools, Nathalie
Issue Date: 2016
Publisher: MARY ANN LIEBERT, INC
Citation: VIRAL IMMUNOLOGY, 29 (3), p. 169-175
Abstract: Morbidity and mortality in the elderly are associated with viral infections, including influenza and cytomegalovirus (CMV). With increasing age, cellular immunity gains importance in protection to influenza, but latent CMV is highly prevalent and associated with immune dysfunction. An insight into the association between immunity against influenza and CMV adds to the understanding of healthy aging. Here we first aimed to measure influenza-specific cellular immunity using granzyme B (GrzB) and interferon-gamma (IFN-gamma)-ELISpot. Next, influenza-specific cellular immunity was associated with humoral and cellular CMV-specific immunity in healthy 65+ elderly. Vaccine trial participants gave additional blood samples 3 weeks after receiving a H1N1 containing vaccine. CMV serology was determined and peripheral blood mononuclear cells were stimulated with influenza N1 or CMV pp65-derived peptide pools for 7 days and rechallenged to assess antigen-specific GgrzB and IFN-gamma responses using ELISpot assays. Results were compared using chi-square and correlation analysis. Eighty-three individuals (60% men, 65% CMV IgG+, age range 65-78y) participated. We found significant positive associations between IFN-gamma and GrzB responses to both influenza and CMV, but also between a positive CMV serostatus and an influenza N1-specific activation marker response (p = 0.013). CMV pp65 responses were detected in CMV IgG+ individuals, but remarkably also in CMV IgG- individuals (27%). In this study, following influenza vaccination, elderly with cellular immunity against CMV were more likely to have cellular immunity against influenza vaccine N1 antigen. These findings stress the need to continue exploring the possible role of CMV in immunosenescence.
Notes: [Theeten, Heidi; Peeters, Kelly; Ogunjimi, Benson; Goossens, Herman; Ieven, Margareta; Van Damme, Pierre; Cools, Nathalie] Univ Antwerp, Fac Med & Hlth Sci, Ctr Evaluat Vaccinat, Vaccine & Infect Dis Inst, Univ Pl 1, B-2610 Antwerp, Belgium. [Mathei, Catharina] Katholieke Univ Leuven, Dept Publ Hlth & Primary Care, Leuven, Belgium. [Ogunjimi, Benson] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat I BIOST, Hasselt, Belgium.
URI: http://hdl.handle.net/1942/21611
DOI: 10.1089/vim.2015.0071
ISI #: 000373237300006
ISSN: 0882-8245
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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