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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21604

Title: Interplay between flow and diffusion in capillary alginate hydrogels
Authors: Schuster, Erich
Sott, Kristin
Strom, Anna
Altskar, Annika
Smisdom, Nick
Geback, Tobias
Loren, Niklas
Hermansson, Anne-Marie
Issue Date: 2016
Citation: SOFT MATTER, 12 (17), p. 3897-3907
Abstract: Alginate gels with naturally occurring macroscopic capillaries have been used as a model system to study the interplay between laminar flow and diffusion of nanometer-sized solutes in real time. Calcium alginate gels that contain homogeneously distributed parallel-aligned capillary structures were formed by external addition of crosslinking ions to an alginate sol. The effects of different flow rates (0, 1, 10, 50 and 100 mu l min(-1)) and three different probes (fluorescein, 10 kDa and 500 kDa fluorescein isothiocyanate-dextran) on the diffusion rates of the solutes across the capillary wall and in the bulk gel in between the capillaries were investigated using confocal laser scanning microscopy. The flow in the capillaries was produced using a syringe pump that was connected to the capillaries via a tube. Transmission electron microscopy revealed an open aggregated structure close to the capillary wall, followed by an aligned network layer and the isotropic network of the bulk gel. The most pronounced effect was observed for the 1 nm-diameter fluorescein probe, for which an increase in flow rate increased the mobility of the probe in the gel. Fluorescence recovery after photobleaching confirmed increased mobility close to the channel, with increasing flow rate. Mobility maps derived using raster image correlation spectroscopy showed that the layer with the lowest mobility corresponded to the anisotropic layer of ordered network chains. The combination of microscopy techniques used in the present study elucidates the flow and diffusion behaviors visually, qualitatively and quantitatively, and represents a promising tool for future studies of mass transport in non-equilibrium systems.
Notes: [Schuster, Erich; Sott, Kristin; Altskar, Annika; Geback, Tobias] SP Tech Res Inst Sweden, Food & Biosci, Gothenburg, Sweden. [Schuster, Erich; Sott, Kristin; Strom, Anna; Altskar, Annika; Geback, Tobias; Loren, Niklas; Hermansson, Anne-Marie] Chalmers, SuMo Biomat, VINN Excellence Ctr, S-41296 Gothenburg, Sweden. [Strom, Anna] Chalmers, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden. [Smisdom, Nick] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Smisdom, Nick] Flemish Inst Technol Res, Environm Risk & Hlth Unit, Mol, Belgium. [Geback, Tobias] Chalmers, Dept Math Sci, S-41296 Gothenburg, Sweden. [Geback, Tobias] Gothenburg Univ, Gothenburg, Sweden. [Hermansson, Anne-Marie] Chalmers, Dept Biol & Biol Engn, S-41296 Gothenburg, Sweden.
URI: http://hdl.handle.net/1942/21604
DOI: 10.1039/c6sm00294c
ISI #: 000374974000008
ISSN: 1744-683X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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