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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21558

Title: Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy
Authors: Bové, Hannelore
Steuwe, Christian
Fron, Eduard
Slenders, Eli
D'Haen, Jan
Fujita, Yasuhiko
Uji-i, Hiroshi
Van de Ven, Martin
Roeffaers, Maarten
Ameloot, Marcel
Issue Date: 2016
Citation: NANO LETTERS, 16 (5), p. 3173-3178
Abstract: Although adverse health effects of carbon black (CB) exposure are generally accepted, a direct, label-free approach for detecting CB particles in fluids and at the cellular level is still lacking. Here, we report nonincandescence related white-light (WL) generation by dry and suspended carbon black particles under illumination with femtosecond (fs) pulsed near-infrared light as a powerful tool for the detection of these carbonaceous materials. This observation is done for four different CB species with diameters ranging from 13 to 500 nm, suggesting this WL emission under fs near-infrared illumination is a general property of CB particles. As the emitted radiation spreads over the whole visible spectrum, detection is straightforward and flexible. The unique property of the described WL emission allows optical detection and unequivocal localization of CB particles in fluids and in cellular environments while simultaneously colocalizing different cellular components using various specific fluorophores as shown here using human lung fibroblasts. The experiments are performed on a typical multiphoton laser-scanning microscopy platform, widely available in research laboratories.
Notes: [Bove, Hannelore; Slenders, Eli; vandeVen, Martin; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Agoralaan Bldg C, B-3590 Diepenbeek, Belgium. [Bove, Hannelore; Steuwe, Christian; Roeffaers, Maarten] Katholieke Univ Leuven, Ctr Surface Chem & Catalysis, Celestijnenlaan 200F, B-3001 Leuven, Belgium. [Fron, Eduard; Fujita, Yasuhiko; Uji-i, Hiroshi] Katholieke Univ Leuven, Dept Chem, Celestijnenlaan 200F, B-3001 Leuven, Belgium. [D'Haen, Jan] Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3950 Diepenbeek, Belgium. [Uji-i, Hiroshi] Hokkaido Univ, Res Inst Elect Sci, Kita Ward, N20W10, Sapporo, Hokkaido 0010020, Japan.
URI: http://hdl.handle.net/1942/21558
DOI: 10.1021/acs.nanolett.6b00502
ISI #: 000375889700042
ISSN: 1530-6984
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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