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Title: Successful Treatment of Human Visceral Leishmaniasis Restores Antigen-Specific IFN-gamma, but not IL-10 Production
Authors: Adem, Emebet
Tajebe, Fitsumbirhan
Getahun, Mulusew
Kiflie, Amare
Diro, Ermias
Hailu, Asrat
Shkedy, Ziv
Mengesha, Bewketu
Mulaw, Tadele
Atnafu, Saba
Deressa, Tekalign
Mathewos, Biniam
Abate, Ebba
Modolell, Manuel
Munder, Markus
Mueller, Ingrid
Takele, Yegnasew
Kropf, Pascale
Issue Date: 2016
Citation: PLoS neglected tropical diseases, 10 (3)
Abstract: One of the key immunological characteristics of active visceral leishmaniasis (VL) is a profound immunosuppression and impaired production of Interferon-gamma (IFN-gamma). However, recent studies from Bihar in India showed using a whole blood assay, that whole blood cells have maintained the capacity to produce IFN-gamma. Here we tested the hypothesis that a population of low-density granulocytes (LDG) might contribute to T cell responses hyporesponsiveness via the release of arginase. Our results show that this population is affected by the anticoagulant used to collect blood: the frequency of LDGs is significantly lower when the blood is collected with heparin as compared to EDTA; however, the anticoagulant does not impact on the levels of arginase released. Next, we assessed the capacity of whole blood cells from patients with active VL to produce IFN-gamma and IL-10 in response to antigen-specific and polyclonal activation. Our results show that whole blood cells produce low or levels below detection limit of IFN-gamma and IL-10, however, after successful treatment of VL patients, these cells gradually regain their capacity to produce IFN-gamma, but not IL-10, in response to activation. These results suggest that in contrast to VL patients from Bihar, India, whole blood cells from VL patients from Gondar, Ethiopia, have lost their ability to produce IFN-gamma during active VL and that active disease is not associated with sustained levels of IL-10 production following stimulation.
Notes: [Adem, Emebet; Tajebe, Fitsumbirhan; Getahun, Mulusew; Kiflie, Amare; Deressa, Tekalign; Mathewos, Biniam; Abate, Ebba] Univ Gondar, Dept Immunol, Gondar, Ethiopia. [Diro, Ermias] Univ Gondar, Dept Internal Med, Gondar, Ethiopia. [Hailu, Asrat] Univ Addis Ababa, Dept Microbiol Immunol & Parasitol, Addis Ababa, Ethiopia. [Shkedy, Ziv] Univ Hasselt, Dept Math & Stat, Diepenbeek, Belgium. [Mengesha, Bewketu; Mulaw, Tadele; Atnafu, Saba; Takele, Yegnasew] Gondar Univ, Leishmaniasis Res & Treatment Ctr, Gondar, Ethiopia. [Modolell, Manuel] Max Planck Inst Immunobiol & Epigenet, Dept Cellular Immunol, Freiburg, Germany. [Munder, Markus] Univ Med Ctr Mainz, Dept Med Hematol Oncol & Pneumol 3, Mainz, Germany. [Mueller, Ingrid; Takele, Yegnasew; Kropf, Pascale] Univ London Imperial Coll Sci Technol & Med, Dept Med, London, England.
URI: http://hdl.handle.net/1942/21541
DOI: 10.1371/journal.pntd.0004468
ISI #: 000373272500016
ISSN: 1935-2735
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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