www.uhasselt.be
DSpace

Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2151

Title: Radial glial cells derived from the neonatal rat spinal cord: morphological and immunocytochemical characterization
Authors: MOREELS, Marjan
VANDENABEELE, Frank
Deryck, L
LAMBRICHTS, Ivo
Issue Date: 2005
Publisher: JAPAN SOC HISTOL DOCUMENTATION NIIGATA UNIV MEDICAL SCHOOL
Citation: ARCHIVES OF HISTOLOGY AND CYTOLOGY, 68(5). p. 361-369
Abstract: Radial glial cells are transiently bipolar cells in the developing central nervous system, best known for their role in guiding migrating neurons. The aim of the present study was to investigate phenotypic characteristics of these bipolar precursor cells in a mixed glial cell culture system derived from the rat neonatal spinal cord. Morphological characterization was assessed by cell-specific immunocytochemical markers (nestin, vimentin, 3CB2) and transmission electron microscopy. Our study yielded substantial evidence showing that the bipolar cells exhibit immunocytochemical and ultrastructural features of radial glial cells. Immunohistochemistry of the neonatal rat spinal cord using the same cell-specific markers suggested these cells are likely derived from the subependymal zone, ventral commissure, and dorsomedial septum. We believe our data recommend this mixed glial culture system to be a valuable tool in studying radial glial cells in vitro.
Notes: Hasselt Univ, Biomedisch Onderzoeksinst, Histol Lab, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, Histol Lab, Diepenbeek, Belgium.Vandenabeele, F, Hasselt Univ, Biomedisch Onderzoeksinst, Histol Lab, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium.frank.vandenabeele@uhasselt.be
URI: http://hdl.handle.net/1942/2151
DOI: 10.1679/aohc.68.361
ISI #: 000235455600003
ISSN: 0914-9465
Category: A1
Type: Journal Contribution
Validation: ecoom, 2007
Appears in Collections: Research publications

Files in This Item:

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.