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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21509

Title: The use of elemental mass spectrometry in phosphoproteomic applications
Authors: Maes, Evelyne
Tirez, Kristof
Baggerman, Geert
Valkenborg, Dirk
Schoofs, Liliane
Ruiz Encinar, Jorge
Mertens, Inge
Issue Date: 2016
Citation: MASS SPECTROMETRY REVIEWS, 35 (3), p. 350-360
Abstract: Reversible phosphorylation is one of the most important post-translational modifications in mammalian cells. Because this molecular switch is an important mechanism that diversifies and regulates proteins in cellular processes, knowledge about the extent and quantity of phosphorylation is very important to understand the complex cellular interplay. Although phosphoproteomics strategies are applied worldwide, they mainly include only molecular mass spectrometry (like MALDI or ESI)-based experiments. Although identification and relative quantification of phosphopeptides is straightforward with these techniques, absolute quantification is more complex and usually requires for specific isotopically phosphopeptide standards. However, the use of elemental mass spectrometry, and in particular inductively coupled plasma mass spectrometry (ICP-MS), in phosphoproteomics-based experiments, allow one to absolutely quantify phosphopeptides. Here, these phosphoproteomic applications with ICP-MS as elemental detector are reviewed. Pioneering work and recent developments in the field are both described. Additionally, the advantage of the parallel use of molecular and elemental mass spectrometry is stressed.
Notes: [Maes, Evelyne; Tirez, Kristof; Baggerman, Geert; Valkenborg, Dirk; Mertens, Inge] Flemish Inst Technol Res VITO, Boeretang 200, B-2400 Mol, Belgium. [Maes, Evelyne; Schoofs, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, Leuven, Belgium. [Baggerman, Geert; Valkenborg, Dirk; Mertens, Inge] Univ Antwerp, CFP CeProMa, B-2020 Antwerp, Belgium. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium. [Ruiz Encinar, Jorge] Univ Oviedo, Dept Phys & Analyt Chem, Oviedo, Spain.
URI: http://hdl.handle.net/1942/21509
DOI: 10.1002/mas.21440
ISI #: 000373733800002
ISSN: 0277-7037
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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