Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21453

Title: Heat-transfer based characterization of DNA on synthetic sapphire chips
Authors: Murib, Mohammed Sharif
Yeap, Weng Siang
Eurlings, Y.
van Grinsven, Bart
Boyen, Hans-Gerhard
Conings, Bert
Michiels, Luc
Ameloot, Marcel
Carleer, Robert
Warmer, J.
Kaul, P.
Haenen, Ken
Schoening, M.J.
De Ceuninck, Ward
Wagner, Patrick
Issue Date: 2016
Citation: SENSORS AND ACTUATORS B-CHEMICAL, 230, p. 260-271
Abstract: In this study, we show that synthetic sapphire (Al2O3), an established implant material, can also serve as a platform material for biosensors comparable to nanocrystalline diamond. Sapphire chips, beads, and powder were first modified with (3-aminopropyl) triethoxysilane (APTES), followed by succinic anhydride (SA), and finally single-stranded probe DNA was EDC coupled to the functionalized layer. The presence of the APTES-SA layer on sapphire powders was confirmed by thermogravimetric analyis and Fourier-transform infrared spectroscopy. Using planar sapphire chips as substrates and X-ray photoelectron spectroscopy (XPS) as surface-sensitive tool, the sequence of individual layers was analyzed with respect to their chemical state, enabling the quantification of areal densities of the involved molecular units. Fluorescence microscopy was used to demonstrate the hybridization of fluorescently tagged target DNA to the probe DNA, including denaturation- and re-hybridization experiments. Due to its high thermal conductivity, synthetic sapphire is especially suitable as a chip material for the heat-transfer method, which was employed to distinguish complementary- and non-complementary DNA duplexes containing single-nucleotide polymorphisms. These results indicate that it is possible to detect mutations electronically with a chemically resilient and electrically insulating chip material.
Notes: Murib, MS (reprint author), Univ Ghent, B-9000 Ghent, Belgium. mohammed.murib@aum.edu.kw
URI: http://hdl.handle.net/1942/21453
DOI: 10.1016/j.snb.2016.02.027
ISI #: 000374329300033
ISSN: 0925-4005
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
published version2.34 MBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.