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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2123

Title: Protein profiling of 3T3-L1 adipocyte differentiation and (tumor necrosis factor alpha-mediated) starvation
Authors: RENES, J.
Bouwman, F
NOBEN, Jean-Paul
EVELO, Chris
ROBBEN, Johan
MARIMAN, E.
Issue Date: 2005
Publisher: BIRKHAUSER VERLAG AG
Citation: CELLULAR AND MOLECULAR LIFE SCIENCES, 62(4). p. 492-503
Abstract: The increased incidence of obesity and related disorders in Western societies requires a thorough understanding of the adipogenic process. Data at the protein level of this process are scarce. Therefore we performed a proteome analysis of differentiating and starving 3T3L1 cells using two-dimensional gel electrophoresis combined with mass spectrometry. Effects of different starvation conditions were examined by subjecting 3T3-L1 adipocytes to caloric restriction, either in the absence or the presence of the lipolysis inducer tumor necrosis factor-alpha. Ninety-three differentially expressed proteins were found during differentiation and starvation of 3T3-L1 cells, 50 of which were identified. GenMAPP/ MAPPfinder software revealed a non-reciprocal regulation of the glycolytic pathway during 3T3-L1 differentiation followed by starvation. Furthermore, proteins involved in growth regulation, cytoskeletal rearrangements and protein modification, 16 of which have not been described before in 3T3-L1 cells, were identified. In conclusion, our data provide valuable information for further understanding of the adipogenic process.
Notes: Maastricht Univ, NUTRIM, Maastricht Proteom Ctr, Dept Human Biol, NL-6200 MD Maastricht, Netherlands. Limburgs Univ Ctr, Biomed Res Inst, B-3590 Diepenbeek, Belgium. Transnatl Univ Limburg, Sch Life Sci, B-3590 Diepenbeek, Belgium. Tech Univ Eindhoven, BiGCaT Bioinformat, NL-6200 MD Maastricht, Netherlands. Maastricht Univ, NL-6200 MD Maastricht, Netherlands.Renes, J, Maastricht Univ, NUTRIM, Maastricht Proteom Ctr, Dept Human Biol, POB 616, NL-6200 MD Maastricht, Netherlands.j.renes@hb.unimaas.nl
URI: http://hdl.handle.net/1942/2123
DOI: 10.1007/s00018-004-4498-9
ISI #: 000227163800009
ISSN: 1420-682X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2006
Appears in Collections: Research publications

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