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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21212

Title: Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media
Authors: Eevers, Nele
Gielen, Marijke
Sanchez-Lopez, A.
Jaspers, Stijn
White, J.C.
Vangronsveld, Jaco
Weyens, Nele
Issue Date: 2015
Citation: Microbial Biotechnology, 8 (4), p. 707-715
Abstract: Many endophytes have beneficial effects on plants and can be exploited in biotechnological applications. Studies hypothesize that only 0.001–1% of all plantassociated bacteria are cultivable. Moreover, even after successful isolations, many endophytic bacteria often show reduced regrowth capacity. This research aimed to optimize isolation processes and culturing these bacteria afterwards. We compared several minimal and complex media in a screening. Beside the media themselves, two gelling agents and adding plant extract to media were investigated to enhance the number and diversity of endophytes as well as the growth capacity when regrown after isolation. In this work, 869 medium delivered the highest numbers of cultivable bacteria, as well as the highest diversity. When comparing gelling agents, no differences were observed in the numbers of bacteria. Adding plant extract to the media lead to a slight increase in diversity. However, when adding plant extract to improve the regrowth capacity, sharp increases of viable bacteria occurred in both rich and minimal media.
URI: http://hdl.handle.net/1942/21212
DOI: 10.1111/1751-7915.12291
ISI #: 000356370600012
ISSN: 1751-7907
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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