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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2121

Title: Characteristics and performance of an immunosorbent assay for human matrix Gla-protein
Authors: SCHURGERS, Jo
Teunissen, KJF
Knapen, MHJ
Kwaijtaal, M
van Diest, R
Ketteler, M
Vermeer, C
Issue Date: 2005
Citation: CLINICA CHIMICA ACTA, 351(1-2). p. 131-138
Abstract: Background: Matrix gammacarboxyglutamate (Gla)-protein (MGP) is a strong inhibitor of soft tissue calcification and is mainly produced by chondrocytes and vascular smooth muscle cells (VSMCs). MGP-deficient mice have extensive calcifications of cartilage and arteries leading to osteopenia, fractures and blood vessel ruptures. Promotor polymorphisms resulting in decreased expression levels were found to be associated with an increased risk for cardiovascular disease in humans. Methods: Recently, an ELISA-based assay has become available with which MGP may be detected in the circulation. The principle of the test kit is that of a competitive immunoassay using a monoclonal antibody against MGP bound to the microtiter plate. Results: Here, we report on a critical evaluation of this assay and its potential diagnostic utility in diseases associated with the degeneration of the arterial vessel wall and cartilage. The biochemical performance of the kit is satisfactory, and significant differences were found between a number of patient cohorts and the reference population. Serum MGP concentrations were significantly decreased in patients with angina pectoris and in various cartilage diseases. Conclusions: The assay allows comparison of groups and may become a suitable marker for risk assessment or diagnosis in cardiovascular disease and osteoarthritis. (C) 2004 Elsevier B.V. All rights reserved.
Notes: Univ Maastricht, Dept Biochem, NL-6200 MD Maastricht, Netherlands. Univ Maastricht, VitaK, NL-6200 MD Maastricht, Netherlands. Univ Hosp Maastricht, Dept Internal Med, Maastricht, Netherlands. Limburgs Univ Ctr, Biomed Res Inst, Diepenbeek, Belgium. Maastricht Univ, EURON, European Grad Sch Neurosci, Maastricht, Netherlands. Univ Hosp Aachen, Dept Nephrol & Clin Immunol, Aachen, Germany. Maastricht Univ, Inst Cardiovasc Res, Maastricht, Netherlands.Vermeer, C, Univ Maastricht, Dept Biochem, POB 616, NL-6200 MD Maastricht, Netherlands.c.vermeer@bioch.unimaas.nl
URI: http://hdl.handle.net/1942/2121
DOI: 10.1016/j.cccn.2004.08.003
ISI #: 000225856000014
ISSN: 0009-8981
Category: A1
Type: Journal Contribution
Validation: ecoom, 2006
Appears in Collections: Research publications

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