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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/21150

Title: High intensity training may reverse the fiber type specific decline in myogenic stem cells in multiple sclerosis patients
Authors: Farup, Jean
Dalgas, Ulrik
Keytsman, Charly
Op 't Eijnde, Bert
Wens, Inez
Issue Date: 2016
Citation: Frontiers in Physiology, 7, (ART N° 193)
Abstract: Multiple sclerosis (MS) is associated with loss of skeletal muscle mass and function. The myogenic stem cells (satellite cells – SCs) are instrumental to accretion of myonuclei, but remain to be investigated in MS. The present study aimed to compare the SC and myonuclei content between MS patients (n=23) and age matched healthy controls (HC, n=18). Furthermore, the effects of 12 weeks of high intensity training on SC and myonuclei content were explored in MS. Muscle biopsies were obtained from m. Vastus Lateralis at baseline (MS+HC) and following 12 weeks of training (MS only). Frozen biopsies were sectioned followed by immunohistochemical analysis for fiber type specific SCs (Pax7+), myonuclei (MN) and central nuclei content and fiber cross-sectional area (fCSA) using ATPase histochemistry. At baseline the SCs per fiber was lower in type II compared to type I fiber in both MS (119%, p<0.01) and HC (69%, p<0.05), whereas the SCs per fCSA was lower in type II fibers compared to type I only in MS (72%, p<0.05). No differences were observed in MN or central nuclei between MS and HC. Following training the type II fiber SCs per fiber and fCSA in MS patients increased by 165% (p<0.05) and 135% (p<0.05), respectively. Furthermore, the type II fiber MN content increased by 35% (p<0.05) following training. In conclusion, the SC content is lower in type II compared to type I fibers in both MS and HC. Furthermore, high intensity training was observed to selectively increase the SC and myonuclei content in type II fibers in MS patients.
Notes: Correspondence: Dr. Inez Wens, Hasselt University, REVAL Rehabilitation Research Center, BIOMED Biomedical Research Institute, Faculty of Medicine and Life Sciences, Diepenbeek, Belgium, inez.wens@uhasselt.be
URI: http://hdl.handle.net/1942/21150
DOI: 10.3389/fphys.2016.00793
ISI #: 000376739000001
ISSN: 1664-042X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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