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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2100

Title: Analysis of bean (Phaseolus vulgaris L.) proteins affected by copper stress
Authors: Cuypers, Ann
Koistinen, Kaisa M.
Kokko, Harri
Karenlampi, Sirpa
Auriola, Seppo
Vangronsveld, Jaco
Issue Date: 2005
Citation: JOURNAL OF PLANT PHYSIOLOGY, 162(4). p. 383-392
Abstract: The effect of excess copper on the expression of soluble proteins in 10-day old Phaseolus vulgaris seedlings was studied with two-dimensional electrophoresis and mass spectrometry, to find sensitive biochemical markers of exposure. Despite major differences in root Cu contents, both 15 and 50 μ M Cu treatments resulted in equal enhancements of Cu in the primary leaves. Three proteins, apparently reacting in a dose-dependent manner to Cu exposure, were identified from roots. The levels of an intracellular pathogenesis-related protein and a newly identified protein homologous to PvPR1, PvPR2, were increased with increasing Cu concentration. The level of a newly identified PR-10 protein decreased in a dose-dependent manner. No significant difference was observed in the leaf protein pattern between controls and 15 μ M Cu-treated plants. However, at 50 μ M Cu exposure, the appearance of PvPR1 and a homologue of Arabidopsis thaliana thylakoid lumenal 17.4 kDa protein was observed. Another protein slightly enhanced by Cu treatment had sequence homology to a mitochondrial precursor of glycine cleavage system H protein of Flaveria pringlei. © 2004 Elsevier GmbH. All rights reserved.
Notes: Limburgs Univ Ctr, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. Univ Kuopio, Dept Biochem, FIN-70211 Kuopio, Finland. Univ Kuopio, Dept Pharmaceut Chem, FIN-70211 Kuopio, Finland.Cuypers, A, Limburgs Univ Ctr, Ctr Environm Sci, Univ Campus, B-3590 Diepenbeek, Belgium.ann.cuypers@luc.ac.be
URI: http://hdl.handle.net/1942/2100
Link to publication: https://www.academia.edu/3109606/Analysis_of_bean_Phaseolus_vulgaris_L._proteins_affected_by_copper_stress
DOI: 10.1016/j.jplph.2004.07.018
ISI #: 000229076100003
ISSN: 0176-1617
Category: A1
Type: Journal Contribution
Validation: ecoom, 2006
Appears in Collections: Research publications

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