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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/2090

Title: Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene
Authors: Taghavi, S
BARAC, Tanja
Greenberg, B
Borremans, B
VANGRONSVELD, Jaco
VAN DER LELIE, Daniel
Issue Date: 2005
Publisher: AMER SOC MICROBIOLOGY
Citation: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12). p. 8500-8505
Abstract: Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Noninoculated plants or plants inoculated with the soil bacterium B. cepacia Bu61 (pTOM-Bu61) were used as controls. Inoculation of poplar had a positive effect on plant growth in the presence of toluene and reduced the amount of toluene released via evapotranspiration. These effects were more dramatic for VM1468, the endophytic strain, than for Bu61. Remarkably, none of the strains became established at detectable levels in the endophytic community, but there was horizontal gene transfer of pTOM-Bu61 to different members of the endogenous endophytic community, both in the presence and in the absence of toluene. This work is the first report of in planta horizontal gene transfer among plant-associated endophytic bacteria and demonstrates that such transfer could be used to change natural endophytic microbial communities in order to improve the remediation of environmental insults.
Notes: Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. Limburgs Univ Ctr, Dept Environm Biol, B-3590 Diepenbeek, Belgium. Vlaamse Instelling Technol Onderzoek, Flemish Inst Technol Res, Environm Technol Expertise Ctr, B-2400 Mol, Belgium.van der Lelie, D, Brookhaven Natl Lab, Dept Biol, Bldg 463, Upton, NY 11973 USA.vdlelied@bnl.gov
URI: http://hdl.handle.net/1942/2090
DOI: 10.1128/AEM.71.12.8500-8505.2005
ISI #: 000234417600106
ISSN: 0099-2240
Category: A1
Type: Journal Contribution
Validation: ecoom, 2007
Appears in Collections: Research publications

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