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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/20692

Title: Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice
Authors: Holvoet, Bryan
Quattrocelli, Mattia
Belderbos, Sarah
Pollaris, Lore
Wolfs, Esther
Gheysens, Olivier
Gijsbers, Rik
Vanoirbeek, Jeroen
Verfaillie, Catherine M.
Sampaolesi, Maurilio
Deroose, Christophe M.
Issue Date: 2015
Publisher: CELL PRESS
Citation: STEM CELL REPORTS, 5 (6), p. 1183-1195
Abstract: Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic Tcells and upregulation of regulatory Tcells.
Notes: [Holvoet, Bryan; Belderbos, Sarah; Gheysens, Olivier; Deroose, Christophe M.] Katholieke Univ Leuven, Ctr Environm & Hlth, Dept Imaging & Pathol Nucl Med & Mol Imaging, B-3000 Leuven, Belgium. [Quattrocelli, Mattia; Sampaolesi, Maurilio] Katholieke Univ Leuven, Ctr Environm & Hlth, Translat Cardiomyol Lab, Dept Dev & Regenerat, B-3000 Leuven, Belgium. [Pollaris, Lore; Vanoirbeek, Jeroen] Katholieke Univ Leuven, Ctr Environm & Hlth, Dept Publ Hlth & Primary Care, B-3000 Leuven, Belgium. [Wolfs, Esther] Univ Hasselt, Histol Lab, Biomed Res Inst, Dept Morphol, B-3590 Diepenbeek, Belgium. [Gijsbers, Rik] Katholieke Univ Leuven, Stem Cell Inst Leuven, Lab Mol Virol & Gene Therapy, Dept Pharmaceut & Pharmacol Sci,Leuven Viral Vect, B-3000 Leuven, Belgium. [Vanoirbeek, Jeroen] Katholieke Univ Leuven, Stem Cell Inst Leuven, Dept Dev & Regenerat, B-3000 Leuven, Belgium.
URI: http://hdl.handle.net/1942/20692
DOI: 10.1016/j.stemcr.2015.10.018
ISI #: 000366142900022
ISSN: 2213-6711
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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