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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/20680

Title: Mitochondrial oxidative DNA damage and exposure to particulate air pollution in mother-newborn pairs
Authors: Grevendonk, Lotte
Janssen, Bram
Vanpoucke, Charlotte
Lefebvre, Wouter
Hoxha, Mirjam
Bollati, Valentina
Nawrot, Tim S.
Issue Date: 2016
Citation: ENVIRONMENTAL HEALTH, 15 (Art N° 10)
Abstract: Background: Studies emphasize the importance of particulate matter (PM) in the formation of reactive oxygen species and inflammation. We hypothesized that PM exposure during different time windows in pregnancy influences mitochondrial 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels, which is an established biomarker for oxidative stress, in both maternal and foetal blood. Methods: We investigated maternal (n = 224) and cord blood (n = 293) from mother-newborn pairs that were enrolled in the ENVIRONAGE birth cohort. We determined mitochondrial 8-OHdG by quantitative polymerase chain reaction (qPCR). Multivariable regression models were used to assess the association between mitochondrial 8-OHdG with PM10 and PM2.5 exposure over various time windows during pregnancy. Results: In multivariable analysis, PM10 exposure during the entire pregnancy was positively associated with levels of mitochondrial 8-OHdG in maternal blood. For an IQR increment in PM10 exposure an increase of 18.3 % (95 % confidence interval (CI): 5.6 to 33.4 %, p = 0.004) in 8-OHdG was observed. PM10 exposure during the last trimester of pregnancy was positively associated with levels of 8-OHdG (28.1, 95 % CI: 8.6 to 51.2 %, p = 0.004, for an IQR increment in PM10). In a similar way, PM2.5 exposure was significantly associated with an increase of mitochondrial 8-OHdG levels in maternal blood during the entire pregnancy (13.9, 95 % CI: 0.4 to 29.4 %, p = 0.04 for an IQR increment in PM2.5 exposure) and third trimester of pregnancy (28.1, 95 % CI: 3.6 to 58.4 %, p = 0.02 for an IQR increment in PM2.5 exposure). In umbilical cord blood, 8-OHdG levels were significantly associated with PM10 exposure during first and second trimester of pregnancy with respectively an increase of 23.0 % (95 % CI: 5.9 to 42.8 %, p = 0.007) and 16.6 % (95 % CI: 1.8 to 33.5 %, p = 0.03) for an IQR increment in PM10 exposure. Conclusions: We found PM-associated increased mitochondrial oxidative DNA damage during pregnancy in both mothers and their newborns. Accordingly, our study showed that particulate air pollution exposure in early life plays a role in increasing systemic oxidative stress, at the level of the mitochondria, both in mother and foetus.
Notes: [Grevendonk, Lotte; Hoxha, Mirjam; Bollati, Valentina] Univ Milan, EPIGET Epidemiol Epigenet & Toxicol Lab, Dept Clin Sci & Community Hlth, Milan, Italy. [Janssen, Bram G.; Nawrot, Tim S.] Hasselt Univ, Ctr Environm Sci, Hasselt, Belgium. [Vanpoucke, Charlotte] Belgian Interreg Environm Agcy, Brussels, Belgium. [Lefebvre, Wouter] Flemish Inst Technol Res VITO, Mol, Belgium. [Nawrot, Tim S.] Leuven Univ, Dept Publ Hlth & Primary Care Occupat & Environm, Leuven, Belgium.
URI: http://hdl.handle.net/1942/20680
DOI: 10.1186/s12940-016-0095-2
ISI #: 000368257400001
ISSN: 1476-069X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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