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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/20675

Title: Cardiac atrial appendage stem cells engraft and differentiate into cardiomyocytes in vivo: A new tool for cardiac repair after MI
Authors: Fanton, Yanick
Robic, Boris
Rummens, Jean-Luc
Daniëls, Annick
Windmolders, Severina
Willems, Leen
Jamaer, Luc
Dubois, Jasperina
Bijnens, Eric
Heuts, Nic
Notelaers, Kristof
Paesen, Rik
Ameloot, Marcel
Mees, Urbain
Bito, Virginie
Declercq, Jeroen
Hensen, Karen
Koninckx, Remco
Hendrikx, Marc
Issue Date: 2015
Abstract: Background: This study assessed whether autologous transplantation of cardiac atrial appendage stem cells (CASCs) preserves cardiac function after myocardial infarction (MI) in a minipig model. Methods and results: CASCs were isolated from right atrial appendages of Gottingen minipigs based on high aldehyde dehydrogenase activity and expanded. MI was induced by a 2 h snare ligation of the left anterior descending coronary artery. Upon reperfusion, CASCs were intramyocardially injected under NOGA guidance (MI-CASC, n = 10). Non-transplanted pigs (MI, n = 8) received sham treatment. 3D electromechanical mapping (EMM) and cardiac MRI were performed to assess left ventricular (LV) function. MI pigs developed LV dilatation at 2 months (2 M), while in the MI-CASC group volumes remained stable. Global LV ejection fraction decreased by 16 +/- 8% inMI animals vs 3 +/- 10% in MI-CASC animals and regional wall thickening in border areas was better preserved in theMI-CASC group. EMM showed decreased viability and wall motion in the LV for both groups POST-MI, whereas at 2 M these parameters only improved in the MI-CASC. Substantial cell retention was accompanied by cardiomyogenic differentiation in 98 +/- 1% of the transplanted CASCs, which functionally integrated. Second harmonic generation microscopy confirmed the formation of mature sarcomeres in transplanted CASCs. Absence of cardiac arrhythmias indicated the safety of CASC transplantation. Conclusion: CASCs preserve cardiac function by extensive engraftment and cardiomyogenic differentiation. Our data indicate the enormous potential of CASCs in myocardial repair. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Notes: [Fanton, Yanick; Rummens, Jean-Luc; Daniels, Annick; Windmolders, Severina; Willems, Leen; Declercq, Jeroen; Hensen, Karen; Koninckx, Remco] Jessa Hosp, Lab Expt Hematol, B-3500 Hasselt, Belgium. [Fanton, Yanick; Robic, Boris; Rummens, Jean-Luc; Windmolders, Severina; Willems, Leen; Notelaers, Kristof; Paesen, Rik; Ameloot, Marcel; Bito, Virginie; Declercq, Jeroen; Hensen, Karen; Koninckx, Remco; Hendrikx, Marc] Hasselt Univ, Fac Med & Life Sci, Hasselt, Belgium. [Robic, Boris; Mees, Urbain; Hendrikx, Marc] Jessa Hosp, Dept Cardiothorac Surg, B-3500 Hasselt, Belgium. [Jamaer, Luc; Dubois, Jasperina] Jessa Hosp, Dept Cardiac Anesthesia, B-3500 Hasselt, Belgium. [Bijnens, Eric; Heuts, Nic] Jessa Hosp, Dept Radiol, MRI Unit, B-3500 Hasselt, Belgium. [Notelaers, Kristof; Paesen, Rik; Ameloot, Marcel; Bito, Virginie] Hasselt Univ, Biomed Res Inst, Hasselt, Belgium.
URI: http://hdl.handle.net/1942/20675
DOI: 10.1016/j.ijcard.2015.07.066
ISI #: 000366144200004
ISSN: 0167-5273
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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