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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/20606

Title: Genetic variation in TLR10 is not associated with chronic Q fever, despite the inhibitory effect of TLR10 on Coxiella burnetii-induced cytokines in vitro
Authors: Ammerdorffer, Anne
Stappers, Mark
Oosting, Marije
Schoffelen, Teske
Hagenaars, Julia C. J. P.
Bleeker-Rovers, Chantal P.
Wegdam-Blans, Marjolijn C.
Wever, Peter C.
Roest, Hendrik-Jan
van de Vosse, Esther
Netea, Mihai G.
Sprong, Tom
Joosten, Leo A. B.
Issue Date: 2016
Publisher: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation: CYTOKINE, 77, p. 196-202
Abstract: Coxiella burnetii, the causative agent of Q fever, is recognized by TLR2. TLR10 can act as an inhibitory receptor on TLR2-derived immune responses. Therefore, we investigated the role of TLR10 on C. burnetii-induced cytokine production and assessed whether genetic polymorphisms in TLR10 influences the development of chronic Q fever. HEK293 cells, transfected with TLR2, TLR10 or TLR2/TLR10, and human peripheral blood mononuclear cells (PBMCs) in the presence of anti-TLR10, were stimulated with C burnetii. In both assays, the absence of TLR10 resulted in increased cytokine responses after C burnetii stimulation. In addition, the effect of single nucleotide polymorphisms (SNPs) in TLR10 was examined in healthy volunteers whose PBMCs were stimulated with C burnetii Nine Mile or the Dutch outbreak isolate C burnetii 3262. Individuals bearing SNPs in TLR10 displayed increased cytokine production upon C burnetii 3262 stimulation. Furthermore, 139 chronic Q fever patients and 220 controls were genotyped for TLR10 N241H, 1775V and I369L. None of these polymorphisms were associated with increased susceptibility to chronic Q fever. In conclusion, TLR10 has an inhibitory effect on in vitro cytokine production by C. burnetii, but the presence of TLR10 polymorphisms does not lead to an increased risk of developing chronic Q fever. (C) 2015 Elsevier Ltd. All rights reserved.
Notes: [Ammerdorffer, Anne; Stappers, Mark H. T.; Oosting, Marije; Schoffelen, Teske; Bleeker-Rovers, Chantal P.; Netea, Mihai G.; Sprong, Tom; Joosten, Leo A. B.] Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 GA Nijmegen, Netherlands. [Ammerdorffer, Anne; Roest, Hendrik-Jan] Cent Vet Inst, Dept Bacteriol & TSEs, NL-8219 PH Lelystad, Netherlands. [Stappers, Mark H. T.] Hasselt Univ, B-3500 Hasselt, Belgium. [Stappers, Mark H. T.; Sprong, Tom] Canisius Wilhelmina Ziekenhuis, Dept Med Microbiol & Infect Dis, NL-6532 SZ Nijmegen, Netherlands. [Hagenaars, Julia C. J. P.] Jeroen Bosch Hosp, Dept Surg, NL-5223 GW Shertogenbosch, Netherlands. [Wegdam-Blans, Marjolijn C.] Lab Pathol & Med Microbiol PAMM, Dept Med Microbiol, NL-5504 DL Veldhoven, Netherlands. [Wever, Peter C.] Jeroen Bosch Hosp, Dept Med Microbiol & Infect Control, NL-5223 GW Shertogenbosch, Netherlands. [van de Vosse, Esther] Leiden Univ, Med Ctr, Dept Infect Dis, NL-2333 ZA Leiden, Netherlands. [Sprong, Tom] Canisius Wilhelmina Ziekenhuis, Dept Internal Med, NL-6532 SZ Nijmegen, Netherlands.
URI: http://hdl.handle.net/1942/20606
DOI: 10.1016/j.cyto.2015.09.005
ISI #: 000366540500025
ISSN: 1043-4666
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

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