Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/20603

Title: Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates
Authors: Ghequire, Maarten G. K.
Dillen, Yörg
Lambrichts, Ivo
Proost, Paul
Wattiez, Ruddy
De Mot, René
Issue Date: 2015
Citation: GENOME BIOLOGY AND EVOLUTION, 7 (10), p. 2810-2828
Abstract: Bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-likeprotein complexesplaying a role ininteractions with eukaryoticorprokaryotic cells. Such tailocins areunable toreplicate inside target cells due to the lack of a phage head with associatedDNA. Asubset of tailocins mediate antagonistic activitieswith bacteriocin-like specificity. Functional characterization of bactericidal tailocins of two Pseudomonas putida rhizosphere isolates revealed not only extensive similarity with the tail assembly module of the Pseudomonas aeruginosa R-type pyocins but also differences in genomic integration site, regulatory genes, and lytic releasemodules. Conversely, these three features are quite similar between strains of the P. putidaandPseudomonasfluorescens clades, althoughphylogenetic analysisof tailgenes suggeststhemtohave evolved separately. Unlike P. aeruginosa R pyocin elements, the tailocin gene clusters of other pseudomonads frequently carry cargo genes, including bacteriocins. Compared with P. aeruginosa, the tailocin tail fiber sequences that act as specificity determinants have divergedmuch more extensively among the other pseudomonad species, mostly isolates from soil and plant environments. Activity of the P. putida antibacterial particles requires a functional lipopolysaccharide layer on target cells, but contrary to R pyocins from P. aeruginosa, strain susceptibilities surpass species boundaries.
Notes: [Ghequire, Maarten G. K.; De Mot, Rene] Univ Leuven, CMPG, Heverlee, Belgium. [Dillen, Yorg; Lambrichts, Ivo] Hasselt Univ, Grp Morphol, Biomed Res Inst BIOMED, Diepenbeek, Leuven, Belgium. [Proost, Paul] Univ Leuven, Rega Inst, Dept Microbiol & Immunol, Lab Mol Immunol, Leuven, Belgium. [Wattiez, Ruddy] Univ Mons, Res Inst Biosci, Prote & Microbiol Lab, B-7000 Mons, Belgium.
URI: http://hdl.handle.net/1942/20603
DOI: 10.1093/gbe/evv184
ISI #: 000364951100002
ISSN: 1759-6653
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
published version1.46 MBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.