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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/20595

Title: Genetic Variation in TLR10, an Inhibitory Toll-Like Receptor, Influences Susceptibility to Complicated Skin and Skin Structure Infections
Authors: Stappers, Mark H. T.
Oosting, Marije
Ioana, Mihai
Reimnitz, Peter
Mouton, Johan W.
Netea, Mihai G.
Gyssens, Inge C.
Joosten, Leo A. B.
Issue Date: 2015
Citation: JOURNAL OF INFECTIOUS DISEASES, 212 (9), p. 1491-1499
Abstract: Background. Toll-like receptors (TLRs) play a central role in the innate immune response to complicated skin and skin structure infections (cSSSIs), with TLR10 being the first family member known to have an inhibitory function. This study assessed the role of TLR10 in recognition of cSSSI-related pathogens and whether genetic variation in TLR10 influences susceptibility to cSSSIs. Methods. Human peripheral blood mononuclear cells (PBMCs) preincubated with anti-TLR10 antibody and HEK-293 cells overexpressing TLRs were exposed to cSSSI pathogens, and cytokine secretion was determined by enzyme-linked immunosorbent assay. A total of 318 patients with cSSSI and 328 healthy controls were genotyped for 4 nonsynonymous single-nucleotide polymorphisms in TLR10, and functional consequences of the TLR10 SNPs were assessed via in vitro stimulation assays. Results. PBMC stimulation with cSSSI pathogens in the presence of TLR10 neutralizing antibody significantly increased interleukin 6 secretion. Overexpression of TLR10 completely abrogated TLR2-induced interleukin 8 secretion by HEK-293 cells in response to cSSSI pathogens. Three polymorphisms in TLR10, I775L, I369L, and N241H, were associated with reduced susceptibility to cSSSIs. The presence of the TLR10 alleles 775L, 369L, or 241H increased interleukin 6 secretion by PBMCs in response to cSSSI pathogens. Conclusions. TLR10 is a modulatory receptor of innate immune responses to cSSSI-related pathogens, and genetic variants in TLR10 are associated with protection against cSSSIs.
Notes: [Stappers, Mark H. T.; Oosting, Marije; Ioana, Mihai; Netea, Mihai G.; Gyssens, Inge C.; Joosten, Leo A. B.] Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 ED Nijmegen, Netherlands. [Mouton, Johan W.] Radboud Univ Nijmegen, Med Ctr, Dept Med Microbiol, NL-6525 ED Nijmegen, Netherlands. [Stappers, Mark H. T.; Mouton, Johan W.; Gyssens, Inge C.] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, Nijmegen, Netherlands. [Mouton, Johan W.] Erasmus MC, Dept Med Microbiol & Infect Dis, Rotterdam, Netherlands. [Stappers, Mark H. T.; Gyssens, Inge C.] Hasselt Univ, Hasselt, Belgium. [Reimnitz, Peter] Bayer Healthcare Pharmaceut, Wuppertal, Germany.
URI: http://hdl.handle.net/1942/20595
DOI: 10.1093/infdis/jiv229
ISI #: 000364767500018
ISSN: 0022-1899
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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