Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/20302

Title: Monitoring the Bystander Killing Effect of Human Multipotent Stem Cells for Treatment of Malignant Brain Tumors
Authors: Leten, Cindy
Trekker, Jesse
Struys, Tom
Roobrouck, Valerie D.
Dresselaers, Tom
Vande Velde, Greetje
Lambrichts, Ivo
Verfaillie, Catherine M.
Himmelreich, Uwe
Issue Date: 2016
Citation: Stem Cells International, 2016
Abstract: Tumor infiltrating stem cells have been suggested as a vehicle for the delivery of a suicide gene towards otherwise difficult to treat tumors like glioma. We have used herpes simplex virus thymidine kinase expressing human multipotent adult progenitor cells in two brain tumor models (hU87 and Hs683) in immune-compromised mice. In order to determine the best time point for the administration of the codrug ganciclovir, the stem cell distribution and viability were monitored in vivo using bioluminescence (BLI) and magnetic resonance imaging (MRI). Treatment was assessed by in vivo BLI and MRI of the tumors. We were able to show that suicide gene therapy using HSV-tk expressing stem cells can be followed in vivo by MRI and BLI. This has the advantage that (1) outliers can be detected earlier, (2) GCV treatment can be initiated based on stem cell distribution rather than on empirical time points, and (3) a more thorough follow-up can be provided prior to and after treatment of these animals. In contrast to rodent stem cell and tumor models, treatment success was limited in our model using human cell lines. This was most likely due to the lack of immune components in the immune-compromised rodents.
Notes: Himmelreich, U (reprint author), Katholieke Univ Leuven, Dept Imaging & Pathol, Biomed MRI, B-3000 Leuven, Belgium. uwe.himmelreich@med.kuleuven.be
URI: http://hdl.handle.net/1942/20302
DOI: 10.1155/2016/4095072
ISI #: 000372970400001
ISSN: 1687-966X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2017
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
published version3.48 MBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.