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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19896

Title: Real-time analysis of dual-display phage immobilization and autoantibody screening using quartz crystal microbalance with dissipation monitoring
Authors: Rajaram, Kaushik
Losada-Perez, Patricia
Vermeeren, Veronique
Hosseinkhani, Baharak
Wagner, Patrick
Somers, Veerle
Michiels, Luc
Issue Date: 2015
Abstract: Over the last three decades, phage display technology has been used for the display of target-specific biomarkers, peptides, antibodies, etc. Phage display-based assays are mostly limited to the phage ELISA, which is notorious for its high background signal and laborious methodology. These problems have been recently overcome by designing a dual-display phage with two different end functionalities, namely, streptavidin (STV)-binding protein at one end and a rheumatoid arthritis-specific autoantigenic target at the other end. Using this dual-display phage, a much higher sensitivity in screening specificities of autoantibodies in complex serum sample has been detected compared to single-display phage system on phage ELISA. Herein, we aimed to develop a novel, rapid, and sensitive dual-display phage to detect autoantibodies presence in serum samples using quartz crystal microbalance with dissipation monitoring as a sensing platform. The vertical functionalization of the phage over the STV-modified surfaces resulted in clear frequency and dissipation shifts revealing a well-defined viscoelastic signature. Screening for autoantibodies using antihuman IgG-modified surfaces and the dual-display phage with STV magnetic bead complexes allowed to isolate the target entities from complex mixtures and to achieve a large response as compared to negative control samples. This novel dual-display strategy can be a potential alternative to the time consuming phage ELISA protocols for the qualitative analysis of serum autoantibodies and can be taken as a departure point to ultimately achieve a point of care diagnostic system.
Notes: [Rajaram, Kaushik; Vermeeren, Veronique; Hosseinkhani, Baharak; Somers, Veerle; Michiels, Luc] Hasselt Univ, Biomed Res Inst BIOMED, B-3500 Hasselt, Belgium. [Losada-Perez, Patricia; Wagner, Patrick] Hasselt Univ, Inst Mat Res IMO, B-3590 Diepenbeek, Belgium. [Losada-Perez, Patricia] IMEC VZW, Div IMOMEC, Diepenbeek, Belgium. [Wagner, Patrick] Katholieke Univ Leuven, Soft Matter & Biophys Sect, Dept Phys & Astron, Leuven, Belgium.
URI: http://hdl.handle.net/1942/19896
DOI: 10.2147/IJN.S84800
ISI #: 000360058000001
ISSN: 1178-2013
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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