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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19895

Title: Early Inflammatory Responses Following Cell Grafting in the CNS Trigger Activation of the Subventricular Zone: A Proposed Model of Sequential Cellular Events
Authors: Praet, Jelle
Santermans, Eva
Daans, Jasmijn
Le Blon, Debbie
Hoornaert, Chloe
Goossens, Herman
Hens, Niel
Van der Linden, Annemie
Berneman, Zwi
Ponsaerts, Peter
Issue Date: 2015
Publisher: COGNIZANT COMMUNICATION CORP
Citation: CELL TRANSPLANTATION, 24 (8), p. 1481-1492
Abstract: While multiple rodent preclinical studies, and to a lesser extent human clinical trials, claim the feasibility, safety, and potential clinical benefit of cell grafting in the central nervous system (CNS), currently only little convincing knowledge exists regarding the actual fate of the grafted cells and their effect on the surrounding environment (or vice versa). Our preceding studies already indicated that only a minor fraction of the initially grafted cell population survives the grafting process, while the surviving cell population becomes invaded by highly activated microglia/macrophages and surrounded by reactive astrogliosis. In the current study, we further elaborate on early cellular and inflammatory events following syngeneic grafting of eGFP(+) mouse embryonic fibroblasts (mEFs) in the CNS of immunocompetent mice. Based on obtained quantitative histological data, we here propose a detailed mathematically derived working model that sequentially comprises hypoxia-induced apoptosis of grafted mEFs, neutrophil invasion, neoangiogenesis, microglia/macrophage recruitment, astrogliosis, and eventually survival of a limited number of grafted mEFs. Simultaneously, we observed that the cellular events following mEF grafting activates the subventricular zone neural stem and progenitor cell compartment. This proposed model therefore further contributes to our understanding of cell graft-induced cellular responses and will eventually allow for successful manipulation of this intervention.
Notes: [Praet, Jelle; Daans, Jasmijn; Le Blon, Debbie; Hoornaert, Chloe; Berneman, Zwi; Ponsaerts, Peter] Univ Antwerp, Expt Cell Transplantat Grp, Lab Expt Hematol, B-2610 Antwerp, Belgium. [Praet, Jelle; Daans, Jasmijn; Le Blon, Debbie; Hoornaert, Chloe; Goossens, Herman; Hens, Niel; Berneman, Zwi; Ponsaerts, Peter] Univ Antwerp, Vaccine & Infect Dis Inst Vaxinfectio, B-2610 Antwerp, Belgium. [Praet, Jelle; Van der Linden, Annemie] Univ Antwerp, BioImaging Lab, B-2610 Antwerp, Belgium. [Santermans, Eva; Hens, Niel] Hasselt Univ, Ctr Stat, I Biostat, Diepenbeek, Belgium. [Hens, Niel] Univ Antwerp, Chermid, B-2610 Antwerp, Belgium.
URI: http://hdl.handle.net/1942/19895
DOI: 10.3727/096368914X682800
ISI #: 000359878900006
ISSN: 0963-6897
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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