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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19862

Title: Both the concentration and redox state of glutathione and ascorbate influence the sensitivity of arabidopsis to cadmium
Authors: Jozefczak, Marijke
Bohler, Sacha
Schat, Henk
Horemans, Nele
Guisez, Yves
Remans, Tony
Vangronsveld, Jaco
Cuypers, Ann
Issue Date: 2015
Citation: ANNALS OF BOTANY, 116 (4), p. 601-612
Abstract: Background and Aims Cadmium (Cd) is a non-essential trace element that elicits oxidative stress. Plants respond to Cd toxicity via increasing their Cd-chelating and antioxidative capacities. They predominantly chelate Cd via glutathione (GSH) and phytochelatins (PCs), while antioxidative defence is mainly based on the use and recycling of both GSH and ascorbate (AsA), complemented by superoxide dismutase (SOD) and catalase (CAT). In addition, both metabolites act as a substrate for the regeneration of other essential antioxidants, which neutralize and regulate reactive oxygen species (ROS). Together, these functions influence the concentration and cellular redox state of GSH and AsA. In this study, these two parameters were examined in plants of Arabidopsis thaliana exposed to sublethal Cd concentrations. Methods Wild-type plants and mutant arabidopsis plants containing 30-45 % of wild-type levels of GSH (cad2-1) or 40-50 % of AsA (vtc1-1), together with the double-mutant (cad2-1 vtc1-1) were cultivated in a hydroponic system and exposed to sub-lethal Cd concentrations. Cadmium detoxification was investigated at different levels including gene expression and metabolite concentrations. Key Results In comparison with wild-type plants, elevated basal thiol levels and enhanced PC synthesis upon exposure to Cd efficiently compensated AsA deficiency in vtc1-1 plants and contributed to decreased sensitivity towards Cd. Glutathione-deficient (cad2-1 and cad2-1 vtc1-1) mutants, however, showed a more oxidized GSH redox state, resulting in initial oxidative stress and a higher sensitivity to Cd. In order to cope with the Cd stress to which they were exposed, GSH-deficient mutants activated multiple alternative pathways. Conclusions Our observations indicate that GSH and AsA deficiency differentially alter plant GSH homeostasis, resulting in opposite Cd sensitivities relative to wild-type plants. Upon Cd exposure, GSH-deficient mutants were hampered in chelation. They experienced phenotypic disturbances and even more oxidative stress, and therefore activated multiple alternative pathways such as SOD, CAT and ascorbate peroxidase, indicating a higher Cd sensitivity. Ascorbate deficiency, however, was associated with enhanced PC synthesis in comparison with wild-type plants after Cd exposure, which contributed to decreased sensitivity towards Cd.
Notes: [Jozefczak, Marijke; Bohler, Sacha; Horemans, Nele; Remans, Tony; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Schat, Henk] Free Univ Amsterdam, Inst Mol & Cellular Biol, NL-1081 HV Amsterdam, Netherlands. [Horemans, Nele] Belgian Nucl Res Ctr, Biosphere Impact Studies, B-2400 Mol, Belgium. [Guisez, Yves] Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium.
URI: http://hdl.handle.net/1942/19862
DOI: 10.1093/aob/mcv075
ISI #: 000361825600012
ISSN: 0305-7364
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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