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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19858

Title: Intracellular localization and dynamics of Hypericin loaded PLLA nanocarriers by image correlation spectroscopy
Authors: Penjweini, Rozhin
Deville, Sarah
D'Olieslaeger, Lien
Berden, Mandy
Ameloot, Marcel
Ethirajan, Anitha
Issue Date: 2015
Publisher: ELSEVIER SCIENCE BV
Citation: JOURNAL OF CONTROLLED RELEASE, 218, p. 82-93
Abstract: The study of cell-nanoparticle interactions is an important aspect for understanding drug delivery using nanocarriers. In this regard, advances in fluorescence based microscopy are useful for the investigation of temporal and spatial behavior of nanoparticles (NPs) within the intracellular environment. In this work, we focus on the delivery of the naturally-occurring hydrophobic photosensitizer Hypericin in human lung carcinoma A549 cells by using biodegradable poly L-lactic acid NPs. For the first time, Hypericin containing NPs are prepared by combining the miniemulsion technique with the solvent evaporation method. This approach yields an efficient loading of the NPs with Hypericin and allows for additional cargo molecules. To monitor the release of Hypercin from the NPs, an additional fluorescent lipophilic dye Coumarin-6 is incorporated in the NPs. Temporal and spatiotemporal image correlation spectroscopy is used to determine the fate of the NPs carrying the potential cargo. Both directed and non-directed motions are detected. By using image cross-correlation spectroscopy and specific fluorescent labeling of endosomes, lysosomes and mitochondria, the dynamics of the cargo loaded NPs in association with the organelles is studied. (C) 2015 Elsevier B.V. All rights reserved.
Notes: [Penjweini, Rozhin; Deville, Sarah; Ameloot, Marcel] Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium. [Deville, Sarah] Flemish Inst Technol Res, Environm Risk & Hlth Unit, Mol, Belgium. [D'Olieslaeger, Lien; Berden, Mandy; Ethirajan, Anitha] Hasselt Univ, IMO IMOMEC, Inst Mat Res, Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/19858
DOI: 10.1016/j.jconrel.2015.09.064
ISI #: 000364167500011
ISSN: 0168-3659
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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