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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19856

Title: Proteomic Alterations in B Lymphocytes of Sensitized Mice in a Model of Chemical-Induced Asthma
Authors: Haenen, Steven
Vanoirbeek, Jeroen A. J.
De Vooght, Vanessa
Schoofs, Liliane
Nemery, Benoit
Clynen, Elke
Hoet, Peter H. M.
Issue Date: 2015
Citation: PLOS ONE, 10 (9)
Abstract: Introduction and Aim The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate (TDI)-sensitized mice into naive mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis (2D-DIGE) to describe the "sensitized signature" of B lymphocytes comparing TDI-sensitized mice with control mice. Results Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses. Conclusion This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function.
Notes: [Haenen, Steven; Vanoirbeek, Jeroen A. J.; De Vooght, Vanessa; Nemery, Benoit; Hoet, Peter H. M.] Katholieke Univ Leuven, Ctr Environm & Hlth, Leuven, Belgium. [Haenen, Steven; Schoofs, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, Leuven, Belgium. [Clynen, Elke] Hasselt Univ, Biomed Res Inst BIOMED, Hasselt, Belgium.
URI: http://hdl.handle.net/1942/19856
DOI: 10.1371/journal.pone.0138791
ISI #: 000361797500109
ISSN: 1932-6203
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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