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|Title: ||Proteomic Alterations in B Lymphocytes of Sensitized Mice in a Model of Chemical-Induced Asthma|
|Authors: ||Haenen, Steven|
Vanoirbeek, Jeroen A. J.
De Vooght, Vanessa
Hoet, Peter H. M.
|Issue Date: ||2015|
|Publisher: ||PUBLIC LIBRARY SCIENCE|
|Citation: ||PLOS ONE, 10 (9)|
|Abstract: ||Introduction and Aim The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate (TDI)-sensitized mice into naive mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis (2D-DIGE) to describe the "sensitized signature" of B lymphocytes comparing TDI-sensitized mice with control mice. Results Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses. Conclusion This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function.|
|Notes: ||[Haenen, Steven; Vanoirbeek, Jeroen A. J.; De Vooght, Vanessa; Nemery, Benoit; Hoet, Peter H. M.] Katholieke Univ Leuven, Ctr Environm & Hlth, Leuven, Belgium. [Haenen, Steven; Schoofs, Liliane] Katholieke Univ Leuven, Res Grp Funct Genom & Prote, Leuven, Belgium. [Clynen, Elke] Hasselt Univ, Biomed Res Inst BIOMED, Hasselt, Belgium.|
|ISI #: ||000361797500109|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2016|
|Appears in Collections: ||Research publications|
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