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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19837

Title: Evaluation of established human IPSC-derived neurons to model neurodegenerative diseases
Authors: Meneghello, G.
Verheyen, A.
Van Ingen, M.
Kuijlaars, J.
Tuefferd, M.
Van den Wyngaert, I.
Nuydens, R.
Issue Date: 2015
Citation: NEUROSCIENCE, 301, p. 204-212
Abstract: Neurodegenerative diseases are difficult to study due to unavailability of human neurons. Cell culture systems and primary rodent cultures have shown to be indispensable to clarify disease mechanisms and provide insights into gene functions. Nevertheless, it is hard to translate new findings into new medicines. The discovery of human induced pluripotent stem cells (iPSC) might partially overcome this problem. Commercially available human iPSC-derived neurons, when thoroughly characterized and suitable for viral transduction, might represent a faster model for drugs screening than the time-consuming derivation and differentiation of iPSC from patient samples. In this study we show that iCell (R) neurons are primarily immature GABAergic neurons within the tested time frame. Addition of C6 glioma conditioned medium improved the bursting frequency of cells without further maturation or evidence for glutamatergic responses. Furthermore, cells were suitable for lentiviral transduction within the tested time frame. Altogether, iCell (R) neurons might be useful to model neurodegenerative diseases in which young GABAergic subtypes are affected. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
Notes: [Meneghello, G.; Verheyen, A.; Van Ingen, M.; Tuefferd, M.; Van den Wyngaert, I.; Nuydens, R.] Janssen Pharmaceut NV, Div A, Janssen Res & Dev, B-2340 Beerse, Belgium. [Kuijlaars, J.] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/19837
DOI: 10.1016/j.neuroscience.2015.05.071
ISI #: 000357877800018
ISSN: 0306-4522
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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