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Title: Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation
Authors: Pirotte, Nicky
Stevens, An-Sofie
Fraguas, Susanna
Plusquin, Michelle
Van Roten, Andromeda
Van Belleghem, Frank
Paesen, Rik
Ameloot, Marcel
Cebrià, Francesc
Artois, Tom
Smeets, Karen
Issue Date: 2015
Citation: Oxidative Medicine and Cellular Longevity, 2015
Abstract: Recent research highlighted the impact of ROS as upstream regulators of tissue regeneration. We investigated their role and targeted processes during the regeneration of different body structures using the planarian Schmidtea mediterranea, an organism capable of regenerating its entire body, including its brain. The amputation of head and tail compartments induces a ROS burst at the wound site independently of the orientation. Inhibition of ROS production by diphenyleneiodonium (DPI) or apocynin (APO) causes regeneration defaults at both the anterior and posterior wound sites, resulting in reduced regeneration sites (blastemas) and improper tissue homeostasis. ROS signaling is necessary for early differentiation and inhibition of the ROS burst results in defects on the regeneration of the nervous system and on the patterning process. Stem cell proliferation was not affected, as indicated by histone H3-P immunostaining, fluorescence-activated cell sorting (FACS), in situ hybridization of smedwi-1, and transcript levels of proliferation-related genes. We showed for the first time that ROS modulate both anterior and posterior regeneration in a context where regeneration is not limited to certain body structures. Our results indicate that ROS are key players in neuroregeneration through interference with the differentiation and patterning processes.
Notes: Correspondence should be addressed to Karen Smeets; karen.smeets@uhasselt.be
URI: http://hdl.handle.net/1942/19700
DOI: 10.1155/2015/392476
ISI #: 000357202200001
ISSN: 1942-0900
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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